园艺与种苗
園藝與種苗
완예여충묘
Rain Fed Crops
2014年
2期
47-49,53
,共4页
陈卫雪%石开明%李闯%周梦莹%杨秋玲%池政松
陳衛雪%石開明%李闖%週夢瑩%楊鞦玲%池政鬆
진위설%석개명%리틈%주몽형%양추령%지정송
空心李%组织培养%快速繁殖
空心李%組織培養%快速繁殖
공심리%조직배양%쾌속번식
Prunus salicina%Tissue culture%Rapid propagation
[目的]探索空心李实生苗高效再生系统的培养基类型和培养基配方。[方法]以沿河沙子、香蜜空心、红心道、东门3号4个品种的空心李为试验材料，研究空心李组织培养过程中的一些关键技术措施。以4种李成年嫁接树梢尖、带芽茎段为外植体，研究空心李嫁接树营养器官组织培养的主要调控因素及控制外植体微生物污染的措施，建立完整、高效、实用的空心李组织培养快速繁殖体系。[结果]研究结果表明，春季生长的植物体内存在大量生长素、细胞分裂素和赤霉素等生长调节物质，因而组织培养时成活率高、生长快；不同条件的培养结果不同，空心李种子发苗的最佳方案是成熟种子在半固体1/2MS萌发后，转至固体MS上培养；不同外植体的培养结果是带腋芽的茎段繁殖系数大于茎尖，差异达到显著水平；在激素配比中，细胞分裂素6-BA与生长素NAA浓度配比适当时有利于丛生芽的诱导和分化。[结论]最适合空心李幼穗的6-BA浓度为0.5 mg/L，而健壮梢则为1.0 mg/L；NAA浓度以1.0 mg/L为好。
[目的]探索空心李實生苗高效再生繫統的培養基類型和培養基配方。[方法]以沿河沙子、香蜜空心、紅心道、東門3號4箇品種的空心李為試驗材料，研究空心李組織培養過程中的一些關鍵技術措施。以4種李成年嫁接樹梢尖、帶芽莖段為外植體，研究空心李嫁接樹營養器官組織培養的主要調控因素及控製外植體微生物汙染的措施，建立完整、高效、實用的空心李組織培養快速繁殖體繫。[結果]研究結果錶明，春季生長的植物體內存在大量生長素、細胞分裂素和赤黴素等生長調節物質，因而組織培養時成活率高、生長快；不同條件的培養結果不同，空心李種子髮苗的最佳方案是成熟種子在半固體1/2MS萌髮後，轉至固體MS上培養；不同外植體的培養結果是帶腋芽的莖段繁殖繫數大于莖尖，差異達到顯著水平；在激素配比中，細胞分裂素6-BA與生長素NAA濃度配比適噹時有利于叢生芽的誘導和分化。[結論]最適閤空心李幼穗的6-BA濃度為0.5 mg/L，而健壯梢則為1.0 mg/L；NAA濃度以1.0 mg/L為好。
[목적]탐색공심리실생묘고효재생계통적배양기류형화배양기배방。[방법]이연하사자、향밀공심、홍심도、동문3호4개품충적공심리위시험재료，연구공심리조직배양과정중적일사관건기술조시。이4충리성년가접수소첨、대아경단위외식체，연구공심리가접수영양기관조직배양적주요조공인소급공제외식체미생물오염적조시，건립완정、고효、실용적공심리조직배양쾌속번식체계。[결과]연구결과표명，춘계생장적식물체내존재대량생장소、세포분렬소화적매소등생장조절물질，인이조직배양시성활솔고、생장쾌；불동조건적배양결과불동，공심리충자발묘적최가방안시성숙충자재반고체1/2MS맹발후，전지고체MS상배양；불동외식체적배양결과시대액아적경단번식계수대우경첨，차이체도현저수평；재격소배비중，세포분렬소6-BA여생장소NAA농도배비괄당시유리우총생아적유도화분화。[결론]최괄합공심리유수적6-BA농도위0.5 mg/L，이건장소칙위1.0 mg/L；NAA농도이1.0 mg/L위호。
The aim was to establish a optimal medium type system, optimum medium for efficient regeneration of Prunus salicina seedlings . [Method]Taking mature embryos of Yanhe plum, Xiangmikongxin plum, Honxindao plum and Dongmen3 plum as materials ,the key technical measures of tissue culture of Prunus salicina were researched. Moreover, according to four grapefruit trees grafted tip and stems with buds as explants, the main regulatory factors of vegetative organs and tissue culture explants and the measures to control microbial contamination were studied. In following that, a complete practical and efficient grapefruit rapid propagation technology system was established. [Result]The results showed that there were a lot of auxin, cytokinins and gibberellins and other growth regulators, and thus tissue culture high survival rate, fast-growing plants growing in spring. What’s more, it presented different results by different culture conditions. The best solution was culturing on MS after the semi-solid 1/2 MS with mature seeds. Culturing results indicated that the axillary buds propagation coefficient greater than the shoot tips of stems and the differences were significant. It also pointed out that 6-BA and NAA concentration ratio was conducive to the induction and differentiation. [Conclusion]This study showed that the most appropriate 6-BA concentration was 0.5mg/L for tender spike, 1.0 mg/L for mature spike, and the NAA concentration was 1.0mg/L as well.
