湖南理工学院学报（自然科学版）
湖南理工學院學報（自然科學版）
호남리공학원학보（자연과학판）
JOURNAL OF HUNAN INSTITUTE OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCE)
2014年
2期
17-20
,共4页
纤维素酶产生菌%筛选%稳定性%抑制剂
纖維素酶產生菌%篩選%穩定性%抑製劑
섬유소매산생균%사선%은정성%억제제
cellulase producing strain%screening%stability%inhibitor
从城市生活垃圾中分离到一株分解纤维素能力最强，并产生胞外酶的菌株，初步确定为芽孢杆菌，并对该菌所产的纤维素酶的酶学性质进行了初步研究.该纤维素酶反应最佳温度为50℃；最适 pH7.0；酶在40~50℃热稳定性较好； CMCase活力在 pH 6.0~7.0处可保持70%以上， FPA酶活在pH 6.0~7.0处可保持79%以上； Ca2+和Mg2+对酶反应表现为明显的促进作用，而Fe3+和Mn2+对酶反应有抑制作用， Na+、Zn2+和K+对酶的影响很小.
從城市生活垃圾中分離到一株分解纖維素能力最彊，併產生胞外酶的菌株，初步確定為芽孢桿菌，併對該菌所產的纖維素酶的酶學性質進行瞭初步研究.該纖維素酶反應最佳溫度為50℃；最適 pH7.0；酶在40~50℃熱穩定性較好； CMCase活力在 pH 6.0~7.0處可保持70%以上， FPA酶活在pH 6.0~7.0處可保持79%以上； Ca2+和Mg2+對酶反應錶現為明顯的促進作用，而Fe3+和Mn2+對酶反應有抑製作用， Na+、Zn2+和K+對酶的影響很小.
종성시생활랄급중분리도일주분해섬유소능력최강，병산생포외매적균주，초보학정위아포간균，병대해균소산적섬유소매적매학성질진행료초보연구.해섬유소매반응최가온도위50℃；최괄 pH7.0；매재40~50℃열은정성교호； CMCase활력재 pH 6.0~7.0처가보지70%이상， FPA매활재pH 6.0~7.0처가보지79%이상； Ca2+화Mg2+대매반응표현위명현적촉진작용，이Fe3+화Mn2+대매반응유억제작용， Na+、Zn2+화K+대매적영향흔소.
Isolated Cellulose decomposing ability was the strongest from city life garbage, and extracellular enzyme production strains, tentatively identified as Bacillus ,and enzymatic properties of cellulase produced by the bacteria were investigated. The cellulase reaction, the optimum temperature is 50℃ ; the optimum pH7.0; enzyme at 40~50℃ has good thermal stability; the activity of CMCase in pH 6 ~ 7 can be maintained above 70%, the activity of FPA in pH 6~7 can be maintained more than 79%; Ca2+ and Mg2+ for the enzyme reaction were increased markedly, While Fe3+ and Mn2+ had inhibitory effect on the enzyme reaction, effect of Na+, Zn2+ and K+ on the enzyme is very small.
