CAJ | 학술논문

【目的】建立金银花药材的 HPLC指纹图谱，并同时测定不同产地商品中绿原酸和木犀草苷的含量，全面评价金银花药材质量。【方法】采用Aglient TC-C18（4?6 mm×250 mm，5μm）色谱柱。采用梯度洗脱，流动相A为1％HAC，流动相B为乙腈，洗脱程序为：0～5 min，A为95％→92％；5～7 min，A为92％→87％；7～28 min，A为87→85％；28～38 min，A为85→84％；38～43 min，A 为84→82％；43～53 min，A 为82→72％；53～63 min，A 为72→50％；63～65 min，A为50→5％；75 min，A为5％。流速为0?8 mL/min；检测波长250 nm；柱温30℃。指纹图谱共有模式的建立采用国家药典委员会“中药色谱指纹图谱相似度评价系统2004 A 版”进行处理分析。【结果】在指纹图谱研究中，标定了15个共有峰，建立了对照指纹图谱，10批药材与共有模式之间相似性良好，相似度均在0?9以上；在所建立的分析条件下，绿原酸、木犀草苷均达到了基线分离，且两者在线性范围内线性关系良好，两种成分的加样回收率分别为99?2％、99?4％。【结论】本法灵敏、准确、简便，可用于金银花药材的综合质量评价。
【목적】건립금은화약재적 HPLC지문도보，병동시측정불동산지상품중록원산화목서초감적함량，전면평개금은화약재질량。【방법】채용Aglient TC-C18（4?6 mm×250 mm，5μm）색보주。채용제도세탈，류동상A위1％HAC，류동상B위을정，세탈정서위：0～5 min，A위95％→92％；5～7 min，A위92％→87％；7～28 min，A위87→85％；28～38 min，A위85→84％；38～43 min，A 위84→82％；43～53 min，A 위82→72％；53～63 min，A 위72→50％；63～65 min，A위50→5％；75 min，A위5％。류속위0?8 mL/min；검측파장250 nm；주온30℃。지문도보공유모식적건립채용국가약전위원회“중약색보지문도보상사도평개계통2004 A 판”진행처리분석。【결과】재지문도보연구중，표정료15개공유봉，건립료대조지문도보，10비약재여공유모식지간상사성량호，상사도균재0?9이상；재소건립적분석조건하，록원산、목서초감균체도료기선분리，차량자재선성범위내선성관계량호，량충성분적가양회수솔분별위99?2％、99?4％。【결론】본법령민、준학、간편，가용우금은화약재적종합질량평개。
Obj ective This study established HPLC chromatographic fingerprint of Lonicerae ja-ponica and measured the contents of chlorogenic acid and galuteolin from different areas?The ob-j ective was to comprehensively evaluate L?japonica quality?[Method]The Aglient TC-C1 8 col-umn (4? 6 mm×250 mm,5μm)and gradient elution was used in this study?The mobile phase was composed of 1% HAC (A)and acetonitrile (B)?The elution processes were shown as fol-lowed:0-5 mins ,A 9 5%→9 2%;5-7 mins ,A 9 2%→8 7%;7-2 8 mins ,A 8 7→8 5%;2 8-3 8 mins,A 85→84%;38-43 mins,A 84→82%;43-53 mins,A 82→72%;53-63 mins,A 72→50%;63-65 mins,A 50→5%;75 min,A 5%?The flow rate was 0? 8 mL/min?The detection wavelength was 250 nm and column temperature of 30 ℃?Data analysis was performed using pro-fessional software named Similarity Evaluation System for Chromatographic Fingerprint of Tradi-tional Chinese Medicine composed by Chinese Pharmacopoeia Committee (Version 2004A)?[Re-sults]Fifteen common peaks were selected as fingerprints of L?japonica there were good similari-ties (>0? 9)between standard fingerprint and the fingerprint from 10 medicinal materials?Under the established conditions,the chlorogenic acid and galuteolin were separated well?Additionally, all calibration curves showed good linear regression and their recovery were 9 9? 2% and 9 9? 4%, respectively?[Conclusion]The method is sensitive,accurate and convenient and it can be used to evaluate L?japonica quality.