CAJ | 학술논문

目的：选择与人类高度相似的大鼠胎盘为研究对象、以血管发育为切入点、经灌胃途径给予妊娠大鼠大、中、小剂量的叶酸干预、选择受精后13.5 d 为时间点，原位、在体观察叶酸对胎盘的作用。方法实验进行时间为2012年9月至2014年1月间，应用免疫细胞化学等技术检测叶酸干预后，胎盘血管内皮生长因子（VEGF）、凝血因子Ⅷ（Ⅷ因子）的表达及游离雌三醇（FE3）的水平。结果叶酸可能通过影响细胞增殖正负向调节因素 VEGF 和Ⅷ因子的表达，促进妊娠大鼠胎盘细胞的增殖活性。叶酸还通过提高胎盘的合成功能，促进胎盘的发育。结论一定剂量的叶酸对胎盘组织结构发育及功能发挥起到有益作用。
목적：선택여인류고도상사적대서태반위연구대상、이혈관발육위절입점、경관위도경급여임신대서대、중、소제량적협산간예、선택수정후13.5 d 위시간점，원위、재체관찰협산대태반적작용。방법실험진행시간위2012년9월지2014년1월간，응용면역세포화학등기술검측협산간예후，태반혈관내피생장인자（VEGF）、응혈인자Ⅷ（Ⅷ인자）적표체급유리자삼순（FE3）적수평。결과협산가능통과영향세포증식정부향조절인소 VEGF 화Ⅷ인자적표체，촉진임신대서태반세포적증식활성。협산환통과제고태반적합성공능，촉진태반적발육。결론일정제량적협산대태반조직결구발육급공능발휘기도유익작용。
Objective Choose the rat placenta which are similiar to human beings as the research objects, and choose vascular development as the breakthrough point. Give the rats folic acid through stomach with high dose, medium dose and small dose as intervention. Methods Choose the 13.5 day after fertilization as the time point, observe folic acid on the function of the placenta in situ and in the body. By oral administration of folic acid pregnancy to the rats, to observe the effect of folic acid intervention on placental blood vessels; detect placental VEGF, CD34 expression and FE3 of pregnant rats level. Results The folic could adjust the expression of VEGFand Ⅷ factor positively and negatively with affect cell proliferation, and promote the proliferation activity of the placenta of the pregnancy rats. Folic acid also promote the development of the placenta by improving the synthetic function of placenta. Conclusion Certain dose of folic acid is good for placental tissue structure and function.