CAJ | 학술논문

目的：探讨Toll样受体2（ TLR2）在慢性乙肝孕妇胎盘中的表达及与宫内感染的关系。方法选择HBsAg阳性的慢性乙肝孕妇80例，其中HBeAg阳性组40例，HBeAg阴性组40例，选择同期在我院分娩的正常孕妇20例为对照组，采用ELISA法检测孕妇外周血HBV血清标志物，采用蛋白印迹法检测TLR2分别在3组胎盘中的表达水平，荧光定量PCR法检测3组胎盘中HBV-DNA含量，同时采用ELISA法检测其分娩的新生儿外周血HBsAg，记录新生儿宫内感染率。结果 TLR2在对照组、HBeAg阴性组、HBeAg阳性组孕妇胎盘中表达逐渐降低，组间比较差异均有统计学意义（P＜0．05），胎盘HBV感染率在对照组、HBeAg阴性组、HBeAg阳性组逐渐升高，组间比较差异均有统计学意义（P＜0．05），新生儿HBV宫内感染率在对照组、HBeAg阴性组、HBeAg阳性组逐渐升高，组间比较差异均有统计学意义（P＜0．05）。结论TLR2可能参与了胎盘感染HBV过程，并与HBV宫内感染有着密切的联系。
목적：탐토Toll양수체2（ TLR2）재만성을간잉부태반중적표체급여궁내감염적관계。방법선택HBsAg양성적만성을간잉부80례，기중HBeAg양성조40례，HBeAg음성조40례，선택동기재아원분면적정상잉부20례위대조조，채용ELISA법검측잉부외주혈HBV혈청표지물，채용단백인적법검측TLR2분별재3조태반중적표체수평，형광정량PCR법검측3조태반중HBV-DNA함량，동시채용ELISA법검측기분면적신생인외주혈HBsAg，기록신생인궁내감염솔。결과 TLR2재대조조、HBeAg음성조、HBeAg양성조잉부태반중표체축점강저，조간비교차이균유통계학의의（P＜0．05），태반HBV감염솔재대조조、HBeAg음성조、HBeAg양성조축점승고，조간비교차이균유통계학의의（P＜0．05），신생인HBV궁내감염솔재대조조、HBeAg음성조、HBeAg양성조축점승고，조간비교차이균유통계학의의（P＜0．05）。결론TLR2가능삼여료태반감염HBV과정，병여HBV궁내감염유착밀절적련계。
Objective To study the expression of TLR 2 in placenta of pregnant women with Chronic Hepatitis B and its relationship with intrauterine infection .Methods 80 cases of HBsAg positive pregnant women(HBeAg positive 40 cases,HBeAg negative 40 cases) and 20 cases of normal pregnant women were en-rolled into the experiment .Western blotting method was used to detect TLR 2expression ,Fluorescence quantita-tive PCR method was adopted to detect HBV -DNA and ELISA method was used to assay HBsAg in neonatal pe-ripheral blood .Results Among control group , HBeAg negative and positive groups, the expression of TLR 2 in placenta was gradually reduced ,placental HBV infection rate and neotanal HBV intrauterine infection rate were gradually increased ,and there had significant difference among three groups .Conclusion TLR2 may participate in the placenta infection induced by HBV ,and has close relationship with HBV intrauterine infection .