CAJ | 학술논문

目的：调查292名马鞍山地区汉族无关个体15个STR基因座的等位基因类型及其频率。方法：用硅珠法提取DNA，采用AmpFISTR Identifiler荧光标记复合扩增系统进行复合扩增；扩增产物用ABI3130 xl型遗传分析仪检测，得到STR分型结果后统计15个基因座的基因频率。结果：15个STR基因座，累计个人识别率达0．999999999999999985，累计非父排除率为0．99999896。结论：该系统在马鞍山地区汉族人群中具有高度多态性，适用于法医学亲权鉴定、个体识别以及DNA数据库的建立。
목적：조사292명마안산지구한족무관개체15개STR기인좌적등위기인류형급기빈솔。방법：용규주법제취DNA，채용AmpFISTR Identifiler형광표기복합확증계통진행복합확증；확증산물용ABI3130 xl형유전분석의검측，득도STR분형결과후통계15개기인좌적기인빈솔。결과：15개STR기인좌，루계개인식별솔체0．999999999999999985，루계비부배제솔위0．99999896。결론：해계통재마안산지구한족인군중구유고도다태성，괄용우법의학친권감정、개체식별이급DNA수거고적건립。
Objective:To investigate the alleles and their frequencies of 15 short tandem repeates(STR) loci from 292 unrelated individuals of Han nation-ality living in Ma′anshan area.Methods:DNA was extracted with silica particles,amplified by PCR with AmpF1STR Identifiler kit,and analyzed with ABI 3130XL Genetic Analyzer.The repeated sequences were statistically summed up for the 15 STRs after genotyping.Results:In the 15 STR loci of Ma′anshan Han population,the total discrimination power(TDP) was 0.999 999 999 999 999 985 and 0.999 998 96 for the combined probabilities of paternity exclu-sion.Conclusion:The 15 STR loci has higher genetic polymorphism in the Han population in Ma′anshan area,and may be applied to paternity testing,in-dividual identification and DNA database reference.