CAJ | 학술논문

目的：制备CL2 MDP（ Disodium clodronate ，氯屈膦酸二钠）脂质体，并研究其包封率和稳定性。方法：采用逆相蒸发法制备CL2 MDP脂质体，在透射电镜下观察脂质体颗粒的形态大小，应用离心法测定CL2 MDP的包封率，并用反复冻融法考察脂质体的稳定性。结果：制备的脂质体形态较为规则，近似呈圆球形，平均直径（200±8）nm，计算测得包封率达（18．6±2．8）％，经冻融处理后脂质体包封率有一定程度的提高。结论：逆相蒸发法可用于制备包封率较高及稳定性良好的CL2 MDP脂质体。
목적：제비CL2 MDP（ Disodium clodronate ，록굴련산이납）지질체，병연구기포봉솔화은정성。방법：채용역상증발법제비CL2 MDP지질체，재투사전경하관찰지질체과립적형태대소，응용리심법측정CL2 MDP적포봉솔，병용반복동융법고찰지질체적은정성。결과：제비적지질체형태교위규칙，근사정원구형，평균직경（200±8）nm，계산측득포봉솔체（18．6±2．8）％，경동융처리후지질체포봉솔유일정정도적제고。결론：역상증발법가용우제비포봉솔교고급은정성량호적CL2 MDP지질체。
Objective:To investigate the approaches to preparing disodium clodronate ( CL2 MDP) liposome and assess its encapsulation efficiency and sta-bility.Methods:Reverse-phase evaporation technique was used to prepare the CL2 MDP liposomes that were characterized under the transmission electron microscope for the aspects.The encapsulation efficiency and stability of the liposomes were determined by ultracentrifugation and repeated freeze-thaw test, respectively.Results:The prepared liposomes exhibited regular aspects with spherical shape.The diameter measured was (200 ±8)nm on average,and the encapsulation efficiency reached (18.6 ±2.8)%.After repeated freeze and thaw treatment,the encapsulation efficiency was increased to some extent. Conclusion: Reverse-phase evaporation appears preferable option to preparation of disodium clodronate liposome ,and contributes to higher encapsulation efficiency and stability .