上海农业学报
上海農業學報
상해농업학보
ACTA AGRICULTURAE SHANGHAI
2014年
3期
102-105
,共4页
黄玉人%赵杰%唐赵莲%支月娥
黃玉人%趙傑%唐趙蓮%支月娥
황옥인%조걸%당조련%지월아
稻恶苗病%串珠镰孢%生物学特性%生化特性
稻噁苗病%串珠鐮孢%生物學特性%生化特性
도악묘병%천주렴포%생물학특성%생화특성
Bakanae disease of rice%Fusarium moniliforme%Biological characteristics%Biochemical property
研究了水稻恶苗病菌(串珠镰孢)的生物学特性,结果表明:麦芽糖琼脂培养基和察贝克氏培养基最适合病菌菌丝的生长,PSA 培养基最适宜孢子的产生;菌丝生长的适宜温度为5~35℃(最适30℃),适宜 pH为6~10,菌落产孢的最适 pH 为8;菌丝和孢子的致死温度为50℃;光照条件对菌落生长影响不明显,但以全暗条件下产孢量最大;在测试的碳源中,鼠李糖、葡萄糖和蔗糖最适宜菌丝生长,甘露醇和淀粉最适宜产孢;氮源中以硝酸钾最适宜菌丝生长,尿素最适宜产孢。生理生化特性测定结果表明:水稻恶苗病菌对孔雀石绿不敏感,对淀粉和硝态氮的利用能力较强。
研究瞭水稻噁苗病菌(串珠鐮孢)的生物學特性,結果錶明:麥芽糖瓊脂培養基和察貝剋氏培養基最適閤病菌菌絲的生長,PSA 培養基最適宜孢子的產生;菌絲生長的適宜溫度為5~35℃(最適30℃),適宜 pH為6~10,菌落產孢的最適 pH 為8;菌絲和孢子的緻死溫度為50℃;光照條件對菌落生長影響不明顯,但以全暗條件下產孢量最大;在測試的碳源中,鼠李糖、葡萄糖和蔗糖最適宜菌絲生長,甘露醇和澱粉最適宜產孢;氮源中以硝痠鉀最適宜菌絲生長,尿素最適宜產孢。生理生化特性測定結果錶明:水稻噁苗病菌對孔雀石綠不敏感,對澱粉和硝態氮的利用能力較彊。
연구료수도악묘병균(천주렴포)적생물학특성,결과표명:맥아당경지배양기화찰패극씨배양기최괄합병균균사적생장,PSA 배양기최괄의포자적산생;균사생장적괄의온도위5~35℃(최괄30℃),괄의 pH위6~10,균락산포적최괄 pH 위8;균사화포자적치사온도위50℃;광조조건대균락생장영향불명현,단이전암조건하산포량최대;재측시적탄원중,서리당、포도당화자당최괄의균사생장,감로순화정분최괄의산포;담원중이초산갑최괄의균사생장,뇨소최괄의산포。생리생화특성측정결과표명:수도악묘병균대공작석록불민감,대정분화초태담적이용능력교강。
The study showed that malt agar and the Czapek's medium were the best for the mycelial growth of Fusarium moniliforme and the PSA medium was the best for the spore production;The suit-able conditions for the mycelial growth were 5 -35 ℃(the optimum temperature was 30 ℃)and pH 6-10,and the optimum pH was 8 for the spore production;The lethal temperature of the mycelium and spore was 50 ℃;Light condition had less effect on the colony growth,but the spore production was the maximum in darkness;As carbon sources,rhamnose,dextrose and saccharose were the most fa-vorable for the mycelial growth,and mannitol and amylum were the most favorable for the spore pro-duction;As nitrogen sources,niter was the most favorable for the mycelial growth,and carbamide was the most favorable for the spore production.The determination of the physiological and biochemical characteristics showed that F .moniliforme was insensitive to malachite green and had a high ability to utilize starch and nitrate nitrogen.