中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2012年
24期
3681-3682
,共2页
纪新梅%李海珠%杜辉%仇思武%赵柏良
紀新梅%李海珠%杜輝%仇思武%趙柏良
기신매%리해주%두휘%구사무%조백량
乙肝病毒外膜大蛋白%肝炎病毒,乙型%病毒复制
乙肝病毒外膜大蛋白%肝炎病毒,乙型%病毒複製
을간병독외막대단백%간염병독,을형%병독복제
Hepatitis B large surface protein%Hepatitis B virus%Virus replication
目的 探讨乙型肝炎病毒外膜大蛋白(HBV-LP)与乙型肝炎病毒脱氧核糖核酸(HBV-DNA)的相关性,动态检测两项指标,分析其在诊断乙型肝炎和临床治疗中的意义.方法 采用酶联免疫吸附试验和实时荧光定量聚合酶链反应技术对230例乙型肝炎患者血清标本进行HBV-LP和HBV-DNA平行检测.结果 乙型肝炎病毒感染患者血清HBV-LP含量与HBV-DNA拷贝数间呈正相关(r=0.84,P＜0.01),230例乙肝病毒感染患者血清标本中,HBV-LP阳性率为84.78％,HBV-DNA阳性率为84.35％,两者差异无统计学意义(P＞0.05).55例HBeAg阴性血清标本中,HBV-LP与HBV-DNA的阳性检出率分别为63.63％(35/55)、58.18％(32/55),两者差异无统计学意义(P＞0.05).HBV-DNA阳性194例中,HBV-LP阳性率(82.47％)明显高于HBeAg阳性率(52.06％)(x2=80.3,P＜0.01).结论 HBV-LP及HBV-DNA是判断乙型肝炎病毒感染患者体内病毒复制程度的重要指标,尤其是HBeAg阴性患者体内病毒复制及预后判断有价值的血清学检测指标,为临床诊断及治疗提供可靠的实验室数据.
目的 探討乙型肝炎病毒外膜大蛋白(HBV-LP)與乙型肝炎病毒脫氧覈糖覈痠(HBV-DNA)的相關性,動態檢測兩項指標,分析其在診斷乙型肝炎和臨床治療中的意義.方法 採用酶聯免疫吸附試驗和實時熒光定量聚閤酶鏈反應技術對230例乙型肝炎患者血清標本進行HBV-LP和HBV-DNA平行檢測.結果 乙型肝炎病毒感染患者血清HBV-LP含量與HBV-DNA拷貝數間呈正相關(r=0.84,P＜0.01),230例乙肝病毒感染患者血清標本中,HBV-LP暘性率為84.78％,HBV-DNA暘性率為84.35％,兩者差異無統計學意義(P＞0.05).55例HBeAg陰性血清標本中,HBV-LP與HBV-DNA的暘性檢齣率分彆為63.63％(35/55)、58.18％(32/55),兩者差異無統計學意義(P＞0.05).HBV-DNA暘性194例中,HBV-LP暘性率(82.47％)明顯高于HBeAg暘性率(52.06％)(x2=80.3,P＜0.01).結論 HBV-LP及HBV-DNA是判斷乙型肝炎病毒感染患者體內病毒複製程度的重要指標,尤其是HBeAg陰性患者體內病毒複製及預後判斷有價值的血清學檢測指標,為臨床診斷及治療提供可靠的實驗室數據.
목적 탐토을형간염병독외막대단백(HBV-LP)여을형간염병독탈양핵당핵산(HBV-DNA)적상관성,동태검측량항지표,분석기재진단을형간염화림상치료중적의의.방법 채용매련면역흡부시험화실시형광정량취합매련반응기술대230례을형간염환자혈청표본진행HBV-LP화HBV-DNA평행검측.결과 을형간염병독감염환자혈청HBV-LP함량여HBV-DNA고패수간정정상관(r=0.84,P＜0.01),230례을간병독감염환자혈청표본중,HBV-LP양성솔위84.78％,HBV-DNA양성솔위84.35％,량자차이무통계학의의(P＞0.05).55례HBeAg음성혈청표본중,HBV-LP여HBV-DNA적양성검출솔분별위63.63％(35/55)、58.18％(32/55),량자차이무통계학의의(P＞0.05).HBV-DNA양성194례중,HBV-LP양성솔(82.47％)명현고우HBeAg양성솔(52.06％)(x2=80.3,P＜0.01).결론 HBV-LP급HBV-DNA시판단을형간염병독감염환자체내병독복제정도적중요지표,우기시HBeAg음성환자체내병독복제급예후판단유개치적혈청학검측지표,위림상진단급치료제공가고적실험실수거.
Objective To explore the correlation between hepatitis B virus large surface protein(HBV-LP)and hepatitis B virus deoxyribonucleic acid(HBV-DNA),analyze the clinical significance of detecting the two index dynamically for diagnosis and treatment of hepatitis B.Methods Enzyme linked immunosorbent assay(ELISA)and fluorescent quantitation polymerase chain reaction(FQ-PCR)were used to detect the levels of HBV-LP and HBV-DNA in serum specimen of 230 hepatitis B patients.Results There was a positive correlation between the content of HBV-LP and copy numbers of HBV-DNA in serum of hepatitis B positive cases(r=0.84,P＜0.01).The positive rate was 84.78％ in HBV-LP and 84.35％ in HBV-DNA of 230 HBV positive cases.As a result there was no significant difference(P＞0.05)in this study.Therefore,the positive rate was 63.63 ％(35/55)in HBV-LP and 58.18％(32/55)in HBV-DNA of 55 HBV negative cases.There was also no significant difference(P＞0.05)in this study.However,the positive rate of HBV-LP(82.47％)was higher than HBE(52.06％)in the 194 HBV-DNA positive cases.There was significantly different(P＜0.01).Conclusion HBV-LP and HBV-DNA are the significant index of the degree of hepatitis B virus replication in hepatitis B positive cases,especially in determining the virus replication and prognosis of treatment in HBe Ag negative,providing the reliable laboratory data for the clinical diagnosis and treatment.
