上海农业学报
上海農業學報
상해농업학보
ACTA AGRICULTURAE SHANGHAI
2014年
3期
42-44
,共3页
灵芝%孢子粉%多糖%三萜%腺苷%测定%破壁时间
靈芝%孢子粉%多糖%三萜%腺苷%測定%破壁時間
령지%포자분%다당%삼첩%선감%측정%파벽시간
Ganoderma lucidum%Sporidia%Polysaccharide%Triterpenes%Adenosine%Determination%Sporoderm breaking time
比较了机械法不同破壁时间对灵芝孢子粉破壁率、多糖、脂肪、腺苷含量测定及核苷和三萜类HPLC 图谱测定的影响。结果表明：破壁率有随破壁时间增加呈提高的趋势，但破壁20 min、30 min、50 min 的破壁率无显著性差异；孢子粉中的油脂以及三萜类的成分，只有在破壁后才能被释放，破壁10 min 后已能使这些有效成分充分溶出，而破壁与否及破壁时间对多糖和腺苷含量的测定结果无显著性影响。
比較瞭機械法不同破壁時間對靈芝孢子粉破壁率、多糖、脂肪、腺苷含量測定及覈苷和三萜類HPLC 圖譜測定的影響。結果錶明：破壁率有隨破壁時間增加呈提高的趨勢，但破壁20 min、30 min、50 min 的破壁率無顯著性差異；孢子粉中的油脂以及三萜類的成分，隻有在破壁後纔能被釋放，破壁10 min 後已能使這些有效成分充分溶齣，而破壁與否及破壁時間對多糖和腺苷含量的測定結果無顯著性影響。
비교료궤계법불동파벽시간대령지포자분파벽솔、다당、지방、선감함량측정급핵감화삼첩류HPLC 도보측정적영향。결과표명：파벽솔유수파벽시간증가정제고적추세，단파벽20 min、30 min、50 min 적파벽솔무현저성차이；포자분중적유지이급삼첩류적성분，지유재파벽후재능피석방，파벽10 min 후이능사저사유효성분충분용출，이파벽여부급파벽시간대다당화선감함량적측정결과무현저성영향。
Ganoderma lucidum sporidia were broken by a mechanical method,and the effects of sporoderm breaking time were compared on the broken sporoderm rate and the determination of polysaccharide,fat and adenosine as well as the HPLC patterns of nucleosides and triterpenes.The results indicated that the broken sporoderm rates had a growth trend with the breaking time increasing but were not significantly different when the breaking time was 20 min,30 min and 50 min;The fat and triterpenes in the sporidia could be released only after sporoderm breakage and could be fully dissolved after breaking the sporoderm for 10 min;The determination of polysaccharide and adenosine contents was not significantly influenced by the intact or broken sporoderm and the sporoderm breaking time.
