上海农业学报
上海農業學報
상해농업학보
ACTA AGRICULTURAE SHANGHAI
2014年
3期
6-9
,共4页
王连会%茅文俊%鲍大鹏
王連會%茅文俊%鮑大鵬
왕련회%모문준%포대붕
香菇%尿嘧啶营养缺陷型%5’-氟乳清酸
香菇%尿嘧啶營養缺陷型%5’-氟乳清痠
향고%뇨밀정영양결함형%5’-불유청산
Lentinula edodes%Uracil auxotroph%5’-fluoro-orotic acid
筛选出对5’-FOA 敏感性较高的香菇‘L26’菌株，采用原生质体紫外诱变技术，获得‘L26’菌株18株尿嘧啶营养缺陷型突变菌株。分子验证表明：其中8株菌株的尿嘧啶合成代谢路径发生了基因突变。6个菌株的 pyrF 基因发生突变，2个菌株的 pyrG 基因发生突变。
篩選齣對5’-FOA 敏感性較高的香菇‘L26’菌株，採用原生質體紫外誘變技術，穫得‘L26’菌株18株尿嘧啶營養缺陷型突變菌株。分子驗證錶明：其中8株菌株的尿嘧啶閤成代謝路徑髮生瞭基因突變。6箇菌株的 pyrF 基因髮生突變，2箇菌株的 pyrG 基因髮生突變。
사선출대5’-FOA 민감성교고적향고‘L26’균주，채용원생질체자외유변기술，획득‘L26’균주18주뇨밀정영양결함형돌변균주。분자험증표명：기중8주균주적뇨밀정합성대사로경발생료기인돌변。6개균주적 pyrF 기인발생돌변，2개균주적 pyrG 기인발생돌변。
The ‘L26’strain of L .edodes with higher sensitivity of 5 ’-FOA was selected,and 18 uracil auxotrophic mutant strains were obtained by UV mutagenesis of protoplast.Molecular verification showed that 8 mutant strains had genetic mutations in uracil synthetic metabolic pathways;there were pyrF gene mutations in 6 strains and pyrG gene mutations in 2 strains.
