山西职工医学院学报
山西職工醫學院學報
산서직공의학원학보
JOURNAL OF SHANXI MEDICAL COLLEGE FOR CONTINUING EDUCATION
2014年
3期
6-10
,共5页
大戟二烯醇%高效液相色谱法%甘遂%对药%群药%配伍
大戟二烯醇%高效液相色譜法%甘遂%對藥%群藥%配伍
대극이희순%고효액상색보법%감수%대약%군약%배오
euphol%HPLC%Euphorbia kansui%liquorice%euphorbia kansui Pinellia Decoction%compatibility
目的：比较甘遂单煎、与甘草对煎及在甘遂半夏汤中群煎时大戟二烯醇的含量变化情况。方法：采用高效液相色谱法。色谱柱为Dikma Diamonsil C8柱（200 mm ×4．6 mm，5μm），流动相为乙腈-水（94：6），检测波长为210 nm，室温。结果：大戟二烯醇在1．2~12μg范围内呈现良好的线性，回归方程为y=32180x+2155．4， r=0．9991；单药、对药、群药的平均回收率分别为97．87％、97．57％、97．34％。大戟二烯醇提取量的变化规律是对药﹥群药﹥单药。结论：建立的方法可用于甘遂的配伍变化研究，甘遂中大戟二烯醇的提取量随着配伍发生明显变化。
目的：比較甘遂單煎、與甘草對煎及在甘遂半夏湯中群煎時大戟二烯醇的含量變化情況。方法：採用高效液相色譜法。色譜柱為Dikma Diamonsil C8柱（200 mm ×4．6 mm，5μm），流動相為乙腈-水（94：6），檢測波長為210 nm，室溫。結果：大戟二烯醇在1．2~12μg範圍內呈現良好的線性，迴歸方程為y=32180x+2155．4， r=0．9991；單藥、對藥、群藥的平均迴收率分彆為97．87％、97．57％、97．34％。大戟二烯醇提取量的變化規律是對藥﹥群藥﹥單藥。結論：建立的方法可用于甘遂的配伍變化研究，甘遂中大戟二烯醇的提取量隨著配伍髮生明顯變化。
목적：비교감수단전、여감초대전급재감수반하탕중군전시대극이희순적함량변화정황。방법：채용고효액상색보법。색보주위Dikma Diamonsil C8주（200 mm ×4．6 mm，5μm），류동상위을정-수（94：6），검측파장위210 nm，실온。결과：대극이희순재1．2~12μg범위내정현량호적선성，회귀방정위y=32180x+2155．4， r=0．9991；단약、대약、군약적평균회수솔분별위97．87％、97．57％、97．34％。대극이희순제취량적변화규률시대약﹥군약﹥단약。결론：건립적방법가용우감수적배오변화연구，감수중대극이희순적제취량수착배오발생명현변화。
Objective:To compare the changes of euphol content of euphorbia kansui in euphorbia herbs,anti-drug Licorice-Euphorbia herbs and Euphorbia kansui Pinellia Decoction. Method:HPLC method was adopted,using column of C8(200 mm × 4. 6 mm,5 μm)in Dikma Diamonsil,a mobile phase of acetonitrile-pure water(94:6),and detection at 210 nm with the room temperature to determine the euphol content from the samples. Result:Euphol had a good liner relation ship in 1. 2~12 μg,and regression equation is y=32 180x+2 155. 4,r=0. 999 1. The average re-covery rate of euphorbia herbs was 97 . 87%;the average recovery rate of anti-drug Licorice-Euphorbia herbs was 97 . 57%;the average recovery rate of Euphorbia kansui Pinellia Decoction was 97 . 34%. Euphol content in euphorbia herbs was the lowest;in anti-drug Licorice-Euphorbia herbs was the highest;in euphorbia herbs was in the middle. Conclusion:The method can be used for the research on compatibility changes of euphorbia herbs. The euphol content changes occur obviously by compatibility.
