CAJ | 학술논문

细菌群落结构对湖泊生态系统的物质循环和能量流动起着非常重要的作用，所以微山湖湖泊生态环境中的细菌群落多样性的分析为通过细菌的环境修复功能改善微山湖的生态环境奠定了理论基础。采用了DGGE 和16 S rDNA 建库相结合的方法对微山湖养殖区与非养殖区及其沉积物细菌群落结构进行了研究，并对养殖区和非养殖区细菌的群落结构进行比较。研究的结果表明，养殖区与非养殖区的细菌群落结构存在较大差异，养殖区的细菌种类明显比非养殖区的细菌种类多，养殖区主要分布为：变形菌门(Proteobacte-ria )、疣微菌门(Verrucomicrobia )、绿弯菌门(Chloroflexi )、酸杆菌门(Acidobacteria )、螺旋体门(Spirochae-tes)、绿菌门(Chlorobi)、拟杆菌门(Bacteroidetes)。其中β-变形菌纲(Betaproteobacteria)为主要优势菌群。非养殖区主要分布为：变形菌门(Proteobacteria)、疣微菌门(Verrucomicrobia)、拟杆菌门(Bacteroidetes)，其中β-变形菌纲(Betaproteobacteria)和δ-变形菌纲(Deltaproteobacteria)种类最为丰富。
세균군락결구대호박생태계통적물질순배화능량류동기착비상중요적작용，소이미산호호박생태배경중적세균군락다양성적분석위통과세균적배경수복공능개선미산호적생태배경전정료이론기출。채용료DGGE 화16 S rDNA 건고상결합적방법대미산호양식구여비양식구급기침적물세균군락결구진행료연구，병대양식구화비양식구세균적군락결구진행비교。연구적결과표명，양식구여비양식구적세균군락결구존재교대차이，양식구적세균충류명현비비양식구적세균충류다，양식구주요분포위：변형균문(Proteobacte-ria )、우미균문(Verrucomicrobia )、록만균문(Chloroflexi )、산간균문(Acidobacteria )、라선체문(Spirochae-tes)、록균문(Chlorobi)、의간균문(Bacteroidetes)。기중β-변형균강(Betaproteobacteria)위주요우세균군。비양식구주요분포위：변형균문(Proteobacteria)、우미균문(Verrucomicrobia)、의간균문(Bacteroidetes)，기중β-변형균강(Betaproteobacteria)화δ-변형균강(Deltaproteobacteria)충류최위봉부。
Bacterial plays an important role in the material circulation and energy flow of the ecological environment.The study laid the theoretical foundation of microbiology for improving the ecological envi-ronment of Weishan by the environmental restoration functions of bacterias.Both building a 16S rDNA da-tabase and DGGE are used to study the bacterial diversity of cultural area and uncultured area of Weishan Lake.The results show that:there difference in bacterial community structure between cultured area and uncultured area of Weishan Lake.The bacterial species of cultured area are mainly Proteobacteria,Verru-comicrobia,Chloroflexi,Acidobacteria,Spirochaetes,Chlorobi and Bacteroidetes.β-Proteobacteria were the dominant flora.The bacterial species of uncultured area are mainly Proteobacteria,Verrucomicrobia, Bacteroidetes,and theβ-Proteobacteria andδ-Proteobacteria are the most abundant species.