CAJ | 학술논문

目的：研究氢气对脊髓缺血再灌注损伤的保护作用及其潜在机制。方法新西兰兔随机分为3组：对照组、脊髓缺血再灌注损伤组和氢水治疗组。对照组仅接受暴露，无脊髓缺血再灌注损伤；缺血再灌注组动物采用ZIVIN法建立脊髓缺血再灌注损伤模型，造成脊髓腰骶段缺血35 min后行再灌注；氢水治疗组动物在再灌注前5 min腹腔注射饱和氢盐水（5 mL/kg），再灌注后8 h重复注射。不同时间点检测后肢运动功能。术后72 h取脊髓进行HE染色、TUNEL染色、氧化-抗氧化指标检测及ELISA检测细胞因子。结果含氢生理盐水治疗能显著改善动物神经功能、抑制脊髓神经元凋亡、抑制氧化应激、改善抗氧化能力，同时降低炎症相关细胞因子，从而发挥脊髓保护作用。结论腹腔注射含氢生理盐水通过抗氧化和抗炎对脊髓缺血再灌注损伤发挥保护作用。
목적：연구경기대척수결혈재관주손상적보호작용급기잠재궤제。방법신서란토수궤분위3조：대조조、척수결혈재관주손상조화경수치료조。대조조부접수폭로，무척수결혈재관주손상；결혈재관주조동물채용ZIVIN법건립척수결혈재관주손상모형，조성척수요저단결혈35 min후행재관주；경수치료조동물재재관주전5 min복강주사포화경염수（5 mL/kg），재관주후8 h중복주사。불동시간점검측후지운동공능。술후72 h취척수진행HE염색、TUNEL염색、양화-항양화지표검측급ELISA검측세포인자。결과함경생리염수치료능현저개선동물신경공능、억제척수신경원조망、억제양화응격、개선항양화능력，동시강저염증상관세포인자，종이발휘척수보호작용。결론복강주사함경생리염수통과항양화화항염대척수결혈재관주손상발휘보호작용。
To investigate the protective effect of hydrogen rich saline on the ischemia-reperfusion ( I/R) injury of the spinal cord in rabbits .Methods New Zealand rabbits were randomly assigned into 3 groups:control group, I/R group and hydrogen group .Animals in control group received sham operation;animals in I/R group underwent I/R of the spinal cord (35 min ischemia and 72 h reperfusion) with ZIVIN method; animals in hydrogen group were intraperitoneally treated with hydrogen rich saline at 5 min before reperfusion and 8 h after reperfusion .At different time points , the neurological functions were determined .At 72 h after reperfusion , animals were sacrificed and the spinal cord was collected for HE staining , TUNEL staining , detection of oxidative and anti-oxidative parameters and cytokines .Results Intraperitoneal treatment with hydrogen rich saline could improve the neurological functions , inhibit the apoptosis of neurons of the spinal cord , suppress the oxidative and elevate anti-oxidative capabilities and reduce the inflammation related cytokines .Conclusion Hydrogen rich saline may exert neuroprotection on rabbits undergoing I/R of the spinal cord via anti-oxidation and anti-inflammation.