CAJ | 학술논문

目的：探讨RNA干扰技术沉默STAT3基因调控腺样囊性癌细胞生长的实验研究。方法：人腺样囊性癌生长细胞株ACC 2常规培养，分别采用A 组为空白转染组，B 组转染阴性对照 siRNA （NC），C 组转染特异性的siRNA（siRNA 组）转染进 ACC2，采用 qRT PCR 和 Western blot 法检测转染后 STAT3 mRNA 和蛋白表达水平的变化。 MTT检测转染后腺样囊性癌细胞增殖。结果：ACC2细胞转染STAT3 siRNA 48 h后， STAT3 mRNA和蛋白的表达均受到明显抑制（P＜0．05）。 ACC 2生长72 h 细胞转染后细胞增殖受到抑制，较对照组有显著性差异（P＜0．05）。结论：STAT3基因有可能成为腺样囊性癌细胞生长基因治疗中重要的靶基因。
목적：탐토RNA간우기술침묵STAT3기인조공선양낭성암세포생장적실험연구。방법：인선양낭성암생장세포주ACC 2상규배양，분별채용A 조위공백전염조，B 조전염음성대조 siRNA （NC），C 조전염특이성적siRNA（siRNA 조）전염진 ACC2，채용 qRT PCR 화 Western blot 법검측전염후 STAT3 mRNA 화단백표체수평적변화。 MTT검측전염후선양낭성암세포증식。결과：ACC2세포전염STAT3 siRNA 48 h후， STAT3 mRNA화단백적표체균수도명현억제（P＜0．05）。 ACC 2생장72 h 세포전염후세포증식수도억제，교대조조유현저성차이（P＜0．05）。결론：STAT3기인유가능성위선양낭성암세포생장기인치료중중요적파기인。
To explore the effect of downregulation STAT3 expression on the growth of adenoid cystic carcinoma (ACC). Method: STAT3 siRNA were designed and chemical synthesized, then transfected into the human adenoid cystic carcinoma cell line , qRT- PCR and Western blot assay were used to detect STAT3 mRNA and protein expression level. MTT were used to detect proliferation in ACC2 cell. Result:STAT3 mRNA and protein expression in ACC2 cell transfected with siRNA STAT3 were inhibited significantly (P<0.05) compared with the NC control group.Proliferation in ACC2 cell was inhibited significantly,and apoptosis was induced significantly compared with the NC control group (P<0.05). Conclusion: STAT3 gene may be an important target gene in gene therapy to adenoid cystic carcinoma cancer.