CAJ | 학술논문

为探索高原动物适应低氧环境的特殊机制,采集野生盘羊和欧拉型藏羊颈静脉血液,测定血液指标,并对EPO基因遗传多样性进行分析。通过检测发现藏羊群体部分EPO基因存在6个核苷酸变异。野生盘羊和欧拉型藏羊的核苷酸变异数分别为4个和6个,单倍型变异平均数分别为1.527和1.312,核苷酸多样性分别为0.0011和0.0010,说明藏羊EPO基因比较保守。野生盘羊优势单倍型Hap-4具有提高红细胞数和血红蛋白含量,而降低红细胞平均体积的趋势。红细胞数和血红蛋白含量的增加提高了盘羊血液携氧能力,红细胞平均体积的降低减小了血液黏稠度,有利于减轻心脏负担,促进血液循环,提高氧运输效率,对藏羊适应高海拔地区的高原低氧环境具有积极作用。
위탐색고원동물괄응저양배경적특수궤제,채집야생반양화구랍형장양경정맥혈액,측정혈액지표,병대EPO기인유전다양성진행분석。통과검측발현장양군체부분EPO기인존재6개핵감산변이。야생반양화구랍형장양적핵감산변이수분별위4개화6개,단배형변이평균수분별위1.527화1.312,핵감산다양성분별위0.0011화0.0010,설명장양EPO기인비교보수。야생반양우세단배형Hap-4구유제고홍세포수화혈홍단백함량,이강저홍세포평균체적적추세。홍세포수화혈홍단백함량적증가제고료반양혈액휴양능력,홍세포평균체적적강저감소료혈액점주도,유리우감경심장부담,촉진혈액순배,제고양운수효솔,대장양괄응고해발지구적고원저양배경구유적겁작용。
To identify the special mechanism of animals adapting to high altitude hypoxia, wild argali and Oula Ti-betan sheep jugular blood were collected to measure the blood parameters and analyze, the polymorphism of erythropoietin ( EPO) . There were 6 effective nucleotide variations in EPO of Tibetan sheep populations. In wild argali and Oula Tibetan sheep, the nucleotide variations were 4 and 6, the average haplotype variations were 1. 527 and 1. 312, and the nucleotide diversities were 0. 001 1 and 0. 001 0, respectively, indicating that the Tibetan sheep EPO gene is more conservative. Hap-4, a dominant haplotype in wild argali, tended to own more red blood cells, higher hemoglobin content and lower corpuscular hematocrit, which were responsible for the in-crease of the blood oxygen carrying capacity and the reduc-tion of blood viscosity. This mechanism can help to reduce the workload on heart, promote blood circulation, and im-prove oxygen transport efficiency for Tibetan sheep to adapt to high altitude hypoxic environment.