江苏农业学报
江囌農業學報
강소농업학보
JIANGSU JOURNAL OF AGRICULTURAL SCIENCES
2014年
3期
514-519
,共6页
凌娜%唐进根%殷玉生%张帆%安榆林
凌娜%唐進根%慇玉生%張帆%安榆林
릉나%당진근%은옥생%장범%안유림
触角电位%植物挥发性物质%杨树%分月扇舟蛾%引诱剂
觸角電位%植物揮髮性物質%楊樹%分月扇舟蛾%引誘劑
촉각전위%식물휘발성물질%양수%분월선주아%인유제
electroantennogram%plant volatile%Populus nigra%Clostera anastomosis%attractant
为给植物挥发物引诱剂的开发提供理论依据和基础资料,选取了分月扇舟蛾( Clostera anastomosis)和黑杨为试验对象,利用触角电位技术测试了分月扇舟蛾成虫对11种杨树叶片挥发物及其混配物的触角电位反应。结果表明,雌虫与雄虫触角对杨树挥发物的触角电位( EAG)反应没有显著差异;成虫交配前后触角对杨树挥发物的EAG反应同样没有显著差异。将不同化合物进行混配发现,邻羟基苯甲醛、顺-3-己烯醇和丁(子)香酚相互混配时可增强分月扇舟蛾的触角电位活性。通过测定5种挥发物的5种浓度梯度的触角电位活性,发现成虫对混合物的触角电位反应相对值开始随着化合物浓度的升高而升高,但到达一定浓度后,触角电位反应相对值随着浓度的升高而降低。
為給植物揮髮物引誘劑的開髮提供理論依據和基礎資料,選取瞭分月扇舟蛾( Clostera anastomosis)和黑楊為試驗對象,利用觸角電位技術測試瞭分月扇舟蛾成蟲對11種楊樹葉片揮髮物及其混配物的觸角電位反應。結果錶明,雌蟲與雄蟲觸角對楊樹揮髮物的觸角電位( EAG)反應沒有顯著差異;成蟲交配前後觸角對楊樹揮髮物的EAG反應同樣沒有顯著差異。將不同化閤物進行混配髮現,鄰羥基苯甲醛、順-3-己烯醇和丁(子)香酚相互混配時可增彊分月扇舟蛾的觸角電位活性。通過測定5種揮髮物的5種濃度梯度的觸角電位活性,髮現成蟲對混閤物的觸角電位反應相對值開始隨著化閤物濃度的升高而升高,但到達一定濃度後,觸角電位反應相對值隨著濃度的升高而降低。
위급식물휘발물인유제적개발제공이론의거화기출자료,선취료분월선주아( Clostera anastomosis)화흑양위시험대상,이용촉각전위기술측시료분월선주아성충대11충양수협편휘발물급기혼배물적촉각전위반응。결과표명,자충여웅충촉각대양수휘발물적촉각전위( EAG)반응몰유현저차이;성충교배전후촉각대양수휘발물적EAG반응동양몰유현저차이。장불동화합물진행혼배발현,린간기분갑철、순-3-기희순화정(자)향분상호혼배시가증강분월선주아적촉각전위활성。통과측정5충휘발물적5충농도제도적촉각전위활성,발현성충대혼합물적촉각전위반응상대치개시수착화합물농도적승고이승고,단도체일정농도후,촉각전위반응상대치수착농도적승고이강저。
To provide theoretical basis and information for development of herbivore-induced plant volatiles as at-tractants, Clostera anastomosis were selected to detect the antennal electrophysiological responses to 11 plant volatiles from poplar leaf revealed by electroantennogram ( EAG) . The female and male adults gave similar EAG responses to poplar vola-tiles, and the EAG response of the antennae of adults before and after mating was consistent. The antennal activity of C. anastomosis be enhanced by the mixture of three volatiles, salicylaldehyde, cis-3-hexenol and eugenol. By measurement of the antennl electrophysiological activities of C. anastomosis in response to 5 concentration gradients of 5 volatiles, an in-crease of relative value of EAG response of adults to the mixtures was found as a function of increase in the concentration of the mixture at the beginning, but decreased after wards.