CAJ | 학술논문

以葡萄酒泥酵母为试验材料,采用超声波辅助法分离提取超氧化物歧化酶(SOD).通过单因素试验,研究了料液比、超声波功率、超声波时间和热变性温度对 SOD 比活力的影响,并采用正交试验确定了超声波法分离提取 SOD的最佳工艺参数.结果表明：葡萄酒泥酵母 SOD提取的最佳工艺参数为料液比1∶2.5、超声功率400 W、超声作用时间16 min、热变性温度45℃,在此条件下,分离提取得到的 SOD比活力最高为169.32 U/mg.此方法操作简单,成本低,所得 SOD比活力较高.
이포도주니효모위시험재료,채용초성파보조법분리제취초양화물기화매(SOD).통과단인소시험,연구료료액비、초성파공솔、초성파시간화열변성온도대 SOD 비활력적영향,병채용정교시험학정료초성파법분리제취 SOD적최가공예삼수.결과표명：포도주니효모 SOD제취적최가공예삼수위료액비1∶2.5、초성공솔400 W、초성작용시간16 min、열변성온도45℃,재차조건하,분리제취득도적 SOD비활력최고위169.32 U/mg.차방법조작간단,성본저,소득 SOD비활력교고.
Taking waste wine yeast as the research obj ect,the superoxide dismutase(SOD)was extrac-ted from it by ultrasonic wave.Four extraction parameters including material-liquid ratio,ultrasonic power, ultrasonic time and thermal denaturation temperature were studied by single factor test,and the optimum process parameters were determined by orthogonal test.The results showed that the optimal material-liquid ratio was 1∶2.5,ultrasonic power was 400 W,ultrasonic time was 16 min,thermal denaturation temperature was 45 ℃,under these conditions,the specific activities of SOD was 169.32 U/mg.The process was sim-ple,inexpensive and the specific activities of SOD was high.