河南科技学院学报（自然科学版）
河南科技學院學報（自然科學版）
하남과기학원학보（자연과학판）
JOURNAL OF HENAN INSTITUTE OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCES EDITION)
2014年
3期
13-16
,共4页
柿树%叶绿素%有机溶剂%研磨法%浸泡法
柿樹%葉綠素%有機溶劑%研磨法%浸泡法
시수%협록소%유궤용제%연마법%침포법
persimmon%chlorophyll%organic solvent%grinding method%soaking method
以甜柿与涩柿叶片为试材,采用3种不同有机溶剂作为提取液,比较研究了甜柿和涩柿2种柿树叶片叶绿素含量的测定方法.结果表明：采用研磨法提取叶绿素时,溶剂以体积分数为95%的乙醇最好,甜柿叶片叶绿素总含量、叶绿素a含量和叶绿素b含量分别为1.428、1.086和0.341 mg/g,涩柿叶片叶绿素总含量、叶绿素a含量和叶绿素b含量分别为0.951、0.737和0.213 mg/g,均显著高于体积分数为80%的丙酮和二者的混合液；采用浸泡法提取叶绿素时,以体积分数为80%的丙酮和体积分数为95%的乙醇按体积比1∶1混合的混合液提取效果最好,甜柿叶片叶绿素总含量、叶绿素a含量和叶绿素b含量分别为1.478、1.089和0.388 mg/g,涩柿叶片叶绿素总含量、叶绿素a含量和叶绿素b含量分别为1.180、0.886和0.294 mg/g,均显著高于体积分数为95%的乙醇和体积分数为80%的丙酮.两种叶绿素提取方法比较发现,浸泡法优于研磨法.
以甜柿與澀柿葉片為試材,採用3種不同有機溶劑作為提取液,比較研究瞭甜柿和澀柿2種柿樹葉片葉綠素含量的測定方法.結果錶明：採用研磨法提取葉綠素時,溶劑以體積分數為95%的乙醇最好,甜柿葉片葉綠素總含量、葉綠素a含量和葉綠素b含量分彆為1.428、1.086和0.341 mg/g,澀柿葉片葉綠素總含量、葉綠素a含量和葉綠素b含量分彆為0.951、0.737和0.213 mg/g,均顯著高于體積分數為80%的丙酮和二者的混閤液；採用浸泡法提取葉綠素時,以體積分數為80%的丙酮和體積分數為95%的乙醇按體積比1∶1混閤的混閤液提取效果最好,甜柿葉片葉綠素總含量、葉綠素a含量和葉綠素b含量分彆為1.478、1.089和0.388 mg/g,澀柿葉片葉綠素總含量、葉綠素a含量和葉綠素b含量分彆為1.180、0.886和0.294 mg/g,均顯著高于體積分數為95%的乙醇和體積分數為80%的丙酮.兩種葉綠素提取方法比較髮現,浸泡法優于研磨法.
이첨시여삽시협편위시재,채용3충불동유궤용제작위제취액,비교연구료첨시화삽시2충시수협편협록소함량적측정방법.결과표명：채용연마법제취협록소시,용제이체적분수위95%적을순최호,첨시협편협록소총함량、협록소a함량화협록소b함량분별위1.428、1.086화0.341 mg/g,삽시협편협록소총함량、협록소a함량화협록소b함량분별위0.951、0.737화0.213 mg/g,균현저고우체적분수위80%적병동화이자적혼합액；채용침포법제취협록소시,이체적분수위80%적병동화체적분수위95%적을순안체적비1∶1혼합적혼합액제취효과최호,첨시협편협록소총함량、협록소a함량화협록소b함량분별위1.478、1.089화0.388 mg/g,삽시협편협록소총함량、협록소a함량화협록소b함량분별위1.180、0.886화0.294 mg/g,균현저고우체적분수위95%적을순화체적분수위80%적병동.량충협록소제취방법비교발현,침포법우우연마법.
With sweet persimmon and astringent persimmon leaves as experimental material,the effects of three extraction solvents and two extraction methods on chlorophyll content were studied.The results showed that,when the chlorophyII was extracted by grinding method,the extraction efficiencies by using 95%ethanol alone were better than mixed solvent or 80% acetone alone.The content of total chlorophyll,chlorophyll a,chlorophyll b in sweet persimmon leaves was 1.428 mg/g,1.086 mg/g,and 0.341 mg/g respectively;The content of total chlorophyll,chlorophyll a, chlorophyll b in astringent persimmon leaves was 0.951 mg/g,0.737 mg/g,and 0.213 mg/g respectively.When the chlorophyll was extracted by soaking method,the extraction efficiencies by using mixed solvent were better than 95%ethanol or 80% acetone alone.The content of total chlorophyll,chlorophyll a,chlorophyll b in sweet persimmon leaves was 1.478 mg/g,1.089 mg/g and 0.388 mg/g respectively;The content of total chlorophyll,chlorophyll a,chlorophyll b in astringent persimmon leaves was 1.180 mg/g,0.886 mg/g,and 0.294 mg/g respectively.When the extraction efficiencies by grinding method was compared with that by soaking method,the latter was better.
