CAJ | 학술논문

用聚丙烯酰胺凝胶电泳法研究了不同质量浓度(0、30、60、90、120、150 mg/L)的TiO2纳米颗粒对体长为(8.0±0.2) cm的金鱼Carassius auratus鳃、心、肝胰脏、肾、脑组织中乳酸脱氢酶(LDH)同工酶表达的影响。结果表明：与对照组相比，金鱼各组织中的LDH酶带数及活性均发生明显的变化，酶带数随纳米颗粒浓度的升高而减少， LDH活性呈现纳米颗粒低浓度诱导表达而高浓度抑制表达的变化规律，且在肝胰脏组织中出现C基因的表达。
용취병희선알응효전영법연구료불동질량농도(0、30、60、90、120、150 mg/L)적TiO2납미과립대체장위(8.0±0.2) cm적금어Carassius auratus새、심、간이장、신、뇌조직중유산탈경매(LDH)동공매표체적영향。결과표명：여대조조상비，금어각조직중적LDH매대수급활성균발생명현적변화，매대수수납미과립농도적승고이감소， LDH활성정현납미과립저농도유도표체이고농도억제표체적변화규률，차재간이장조직중출현C기인적표체。
The effects of TiO2 nanoparticle concentrations (0, 30, 60, 90, 120,and 150 mg/L) on expression of lactate dehydrogenase ( LDH) isozymes in gill, heart, hepatopancreas, kidney and brain of goldfish Carassius aura-tus with body length of (8. 0±0. 2)cm by polypropylene-amide erect-board electrophoresis. The results showed that both the activity of LDH isozymes and the number of enzyme bands were found significant changes in all of the tissues in the treated fish compared with the fish in the control group. There were less number of enzyme bands as the TiO2 nanoparticle concentration was increased and the activity of LDH was facilitated at low concentration of TiO2 nanoparticles and inhibited at high concentration of TiO2 nanoparticles. The C gene expression was observed in the hepatopancreas.