CAJ | 학술논문

分别采用热水索氏法和碱水渗漉法提取多棘海盘车Asterias amurensis的多糖组分,将提取的粗多糖经三氯乙酸法脱蛋白、离心透析、冻干,分别得到多糖样品PⅠ、 PⅡ,并分析了多糖PⅠ、 PⅡ在体外对羟基自由基(·OH)、超氧阴离子自由基( O-2·)和DPPH ·的清除能力。结果表明： PⅠ、 PⅡ对· OH、O-2·和DPPH·均具有一定的清除作用,其抗氧化活性均随着多糖浓度的增大逐渐增强。研究表明,采用热水索氏法提取得到的多糖PⅠ对3种自由基的清除率均强于采用碱水渗漉法提取得到的多糖PⅡ。
분별채용열수색씨법화감수삼록법제취다극해반차Asterias amurensis적다당조분,장제취적조다당경삼록을산법탈단백、리심투석、동간,분별득도다당양품PⅠ、 PⅡ,병분석료다당PⅠ、 PⅡ재체외대간기자유기(·OH)、초양음리자자유기( O-2·)화DPPH ·적청제능력。결과표명： PⅠ、 PⅡ대· OH、O-2·화DPPH·균구유일정적청제작용,기항양화활성균수착다당농도적증대축점증강。연구표명,채용열수색씨법제취득도적다당PⅠ대3충자유기적청제솔균강우채용감수삼록법제취득도적다당PⅡ。
The polysaccharides PⅠand PⅡwere extracted from starfish Asterias amurensis by hot water soxhlet ex-traction and alkaline percolation extraction method, and centrifuged, dialyzed and freeze-dried by TDA. The clear-ance of hydroxyl radicals ( ·OH) ,superoxide anion radical ( O-2 · ) and DPPH· radical contents by the polysac-charides PⅠand PⅡwas studied in vitro to evaluate antioxidant activity of the polysaccharides components from the starfish. It was found that the polysaccharides PⅠand PⅡ showed certain clearance capacity of ·OH,O-2 · and DPPH· and the antioxidant activity was enhanced with the elevated polysaccharide concentrations. There were higher free radical clearance rate in the polysaccharide PⅠextracted by soxhlet method of hot water than in the pol-ysaccharide PⅡextracted by alkaline percolation method.