CAJ | 학술논문

对福建省东山县每年6-10月网箱养殖褐篮子鱼Siganus fuscescens暴发的不明原因疾病(死亡率达80%)进行了研究。濒死褐篮子鱼从网箱底层游至水面，主要症状是体色发黑，开始游动快，之后变慢，最后呈现游动失衡状态直至死亡。解剖可见脾脏和肾脏肿大、出血，胃肠空虚，肠道发红。通过对流行病学调查发现，该病具有明显的传染性，从病鱼肝脏、脾脏和肾脏中未分离到致病菌，也未检测到大量寄生虫。对病鱼组织病理切片观察，其肝脏、脾脏和肾脏出现大量肿大细胞。参考OIE设计虹彩病毒的检测引物，以从自然发病鱼的各种组织提取的DNA作为模板，检测结果显示，病鱼的各种组织均能扩增出与预期大小片段相符的特异性产物。经过克隆测序比对显示，扩增条带的基因序列与肿大细胞病毒属的真鲷虹彩病毒( RSIV)、石斑鱼虹彩病毒( GIV )和传染性脾肾坏死病毒( ISKNV )核苷酸序列同源性分别为98.6%、97.4%和93.2%。利用MCP序列构建系统发育树，结果显示，导致褐篮子鱼发病死亡的病毒为肿大细胞病毒属的虹彩病毒，暂命名为褐篮子鱼虹彩病毒( SFIV)。
대복건성동산현매년6-10월망상양식갈람자어Siganus fuscescens폭발적불명원인질병(사망솔체80%)진행료연구。빈사갈람자어종망상저층유지수면，주요증상시체색발흑，개시유동쾌，지후변만，최후정현유동실형상태직지사망。해부가견비장화신장종대、출혈，위장공허，장도발홍。통과대류행병학조사발현，해병구유명현적전염성，종병어간장、비장화신장중미분리도치병균，야미검측도대량기생충。대병어조직병리절편관찰，기간장、비장화신장출현대량종대세포。삼고OIE설계홍채병독적검측인물，이종자연발병어적각충조직제취적DNA작위모판，검측결과현시，병어적각충조직균능확증출여예기대소편단상부적특이성산물。경과극륭측서비대현시，확증조대적기인서렬여종대세포병독속적진조홍채병독( RSIV)、석반어홍채병독( GIV )화전염성비신배사병독( ISKNV )핵감산서렬동원성분별위98.6%、97.4%화93.2%。이용MCP서렬구건계통발육수，결과현시，도치갈람자어발병사망적병독위종대세포병독속적홍채병독，잠명명위갈람자어홍채병독( SFIV)。
An unknown disease with massive mortality of 80% was studied in spinefoot Siganus fuscescens cultured in sea net cages in Dongshan County, Fujian Province from June to October. The morbid spinefoot from the cage bottom floating to the surface showed the symptoms of black body, rapid movement, and then slowstagnant, finally imbalance, even death. The postmortem examination revealed that morbid spinefoot had obviously swollen spleen and kidney with petechial haemorrhages, devoid of stomach, and bloody intestine. Epidemiological survey showed the disease had obvious infectious, and that no pathogenic bacteria and parasites were found from the organs inclu-ding liver, spleen and kidney. Many enlargement and necrosis cells were histopathologically observed in the liver, spleen and kidney. The specific products with predicted size were obtained from all diseased tissues by the primers designed according to the sequence of OIE. Comparative analysis of nucleotide sequences with the GenBank data-bases using Blast database network service indicated that the putative gene products from infected gobies shared 98. 6% identity with red sea bream iridovirus(RSIV), 97. 4% identity with grouper iridovirus(GIV)and 93. 2% i-dentity with infectious spleen and kidney necrosis virus ( ISKNV) . The phylogenetic tree was constructed based on neighbor-joining analyses of the MCP gene sequences, indicating that the pathogen in present study was a member of genus Megalocytivirus, named as tentative Siganus fuscescens iridovirus ( SFIV) .