CAJ | 학술논문

采用16S rDNA克隆文库法对某刺参养殖场患病刺参Apostichopus japonicus幼苗(体长为3~5 cm)表皮的菌群结构进行分析。结果表明：化皮参苗病灶组织和未明显化皮参苗表皮菌群均由γ-变形菌纲、β-变形菌纲和α-变形菌纲组成，优势菌为γ-变形菌纲，主要包括志贺氏菌Shigella sp.、不动杆菌Acineto-bacter sp.和假单胞菌Pseudomonas sp.；化皮参苗病灶组织中志贺氏菌、不动杆菌和假单胞菌比例分别为49%、17%和5%，未明显化皮参苗表皮中3类菌的比例分别为20%、26%、9%。将该养殖场育苗池海水作为对照并进行菌群结构分析，结果显示，水中菌群同样由γ-变形菌纲、β-变形菌纲和α-变形菌纲组成，优势菌也为γ-变形菌纲，主要包含弧菌Vibrio sp.(75%)与志贺氏菌(12%)。此外，对化皮参苗病灶处进行细菌分离培养，得到一株优势菌，经16S rDNA初步鉴定为弧菌。研究表明，采用16S rDNA克隆文库法所得结果与传统的病原分离培养法存在差异。
채용16S rDNA극륭문고법대모자삼양식장환병자삼Apostichopus japonicus유묘(체장위3~5 cm)표피적균군결구진행분석。결과표명：화피삼묘병조조직화미명현화피삼묘표피균군균유γ-변형균강、β-변형균강화α-변형균강조성，우세균위γ-변형균강，주요포괄지하씨균Shigella sp.、불동간균Acineto-bacter sp.화가단포균Pseudomonas sp.；화피삼묘병조조직중지하씨균、불동간균화가단포균비례분별위49%、17%화5%，미명현화피삼묘표피중3류균적비례분별위20%、26%、9%。장해양식장육묘지해수작위대조병진행균군결구분석，결과현시，수중균군동양유γ-변형균강、β-변형균강화α-변형균강조성，우세균야위γ-변형균강，주요포함호균Vibrio sp.(75%)여지하씨균(12%)。차외，대화피삼묘병조처진행세균분리배양，득도일주우세균，경16S rDNA초보감정위호균。연구표명，채용16S rDNA극륭문고법소득결과여전통적병원분리배양법존재차이。
Flora on eipthelia of diseased juvenile sea cucumber Apostichopus japonicus with body length of 3-5 cm showing body wall lesions was studied by a method of 16S rDNA cloning library. The results showed that the bacte-ria were composed of Alphaproteo bacteria, Betaproteo bacteria and Gammaproteo bacteria, with the predominant flora of Shigella sp. , Acinetobacter sp. and Pseudomonas sp. on the lesions of the diseased sea cucumber as well as sea cucumber without obvious symptom. There was 49% of Shigella sp. , 17% of Acinetobacter sp. and 5% of Pseudomonas sp. in the diseased sea cucumber, while there was 20% of Shigella sp. , 26% of Acinetobacter sp. and 9% of Pseudomonas sp. in those without lesions. The members of Alphaproteo bacteria, Betaproteo bacteria and Gammaproteo bacteria were found in the seawater from the culture ponds as a control group, with predominant phaproteobacteria including Vibrio sp. (75%) and Shigella sp. (12%). Also, a predominant bacterium strain was isolated from the diseased cultured sea cucumber by a traditional method based on bacterial culture, and identified as Vibrio sp. by 16S rDNA cloning library. The findings indicate that the predominant flora is varied with the iden-tifying method including a traditional method and 16S rDNA cloning library.