CAJ | 학술논문

建立一种测定葛根超微粉中葛根素含量的方法。采用RP-HPLC法测定葛根素的含量。色谱柱为Agilent SGE protecol C18（5μm 4．6×250mm），流动相为甲醇-水（25∶75），流速为1．0mL/min，柱温为35℃，检测波长为250nm。葛根素在47～470μg/mL的线性关系良好，R＝0．9999。该方法操作简便、稳定可靠，可用于葛根超微粉的葛根素含量测定。
건립일충측정갈근초미분중갈근소함량적방법。채용RP-HPLC법측정갈근소적함량。색보주위Agilent SGE protecol C18（5μm 4．6×250mm），류동상위갑순-수（25∶75），류속위1．0mL/min，주온위35℃，검측파장위250nm。갈근소재47～470μg/mL적선성관계량호，R＝0．9999。해방법조작간편、은정가고，가용우갈근초미분적갈근소함량측정。
This study has been to establish a method of determination of puerarin in roots of Puerarin loba -ta superfine powder.SGE protecol C18 (5μm,4.6 ×250mm) chromatographic column was used ,with mix-tures of methanol and water as mobile phase (25∶75,v/v),the flow rate was 1.0mL/min,column temperature was 35℃,the wavelength was 250nm,the injection volume was 20 μL.The linear correlation of puerarin is good in range of 47~470μg/ml,the correlation coefficient r2 is 0.9998.The presented method is simple and reliable for determining of roots of Puerarin lobata superfine powder .