CAJ | 학술논문

运用同源克隆的方法和RACE技术,从刀鲚(Coilia nasus)肌肉组织中克隆了肌肉生成抑制素基因(Myostatin, MSTN)的 cDNA 全长并分析了肌肉生长抑制素基因在刀鲚不同组织的表达情况。结果表明,刀鲚 MSTN 基因的cDNA全长2252 bp,编码区1125 bp,编码374氨基酸。二级结构预测显示,刀鲚MSTN具有MSTN家族的典型结构域,包含 Pfam 和 TGFB 结构域。运用荧光定量的方法检测了该基因在刀鲚不同组织中的表达。结果显示, MSTN基因在健康刀鲚肌肉和脑中呈高表达,鳃、肝、脾、肠、肾和头肾中微量表达。根据以上研究结果认为,刀鲚 MSTN 基因的序列具有高度保守性,组织表达模式相似,推测该基因的功能也具有高度保守性。本研究旨为MSTN基因在刀鲚后续育种工作提供基础依据。
운용동원극륭적방법화RACE기술,종도제(Coilia nasus)기육조직중극륭료기육생성억제소기인(Myostatin, MSTN)적 cDNA 전장병분석료기육생장억제소기인재도제불동조직적표체정황。결과표명,도제 MSTN 기인적cDNA전장2252 bp,편마구1125 bp,편마374안기산。이급결구예측현시,도제MSTN구유MSTN가족적전형결구역,포함 Pfam 화 TGFB 결구역。운용형광정량적방법검측료해기인재도제불동조직중적표체。결과현시, MSTN기인재건강도제기육화뇌중정고표체,새、간、비、장、신화두신중미량표체。근거이상연구결과인위,도제 MSTN 기인적서렬구유고도보수성,조직표체모식상사,추측해기인적공능야구유고도보수성。본연구지위MSTN기인재도제후속육충공작제공기출의거。
Estuarine tapertail anchovy (Coilia nasus, junior synonym C. ectenes) are widely distributed throughout the Yangtze River, the coastal waters of China and Korea, and the Ariake Sound of Japan. The species is commercially im-portant because of its nutritional value and taste. In recent years, overharvest and changes in the aquatic ecosystem al-most resulted in the extinction of the species in the middle reaches of the Yangtze River. Researchers have evaluated a number of measures to conserve the species, including captive breeding in ponds, artificial propagation, and larval rearing. As a result, the immediate threat to C.nasus has been alleviated at present. However, artificially cultured C. nasus grow relatively slowly (~125 g in 2+years) so there is a need to improve the growth rate. To determine the mo-lecular mechanisms controlling growth and meat quality in Coilia nasus, we cloned the myostatin gene in C. nasus by homologous cloning methods. Its full-length cDNA was 2 252 bp long, with a 1 125 bp open reading frame (ORF) that encoded a 374 amino acid protein. The C. nasusu MSTN protein was predicted to contain a signal peptide sequence, conserved cysteine residues, and RXXR proteolytic sites. Gene expression was deduced by qRT-PCR. The MSTN gene of C. nasus was expressed strongly in the muscle and brain, but weakly in the gills, liver, spleen, intestine, kidney, and head kidney. MSTN expression in C. nasus was not limited to skeletal muscle. Our results suggest that the biological actions of MSTN in C. nasus, and possibly in other fishes, may not be limited to skeletal muscle growth repression, but may also influence different cell types and organ systems, particularly brain cells. The MSTN gene has shown signifi-cant potential in mammalian breeding programs, and our results provide for a basis for development of breeding pro-grams in C. nasus.