CAJ | 학술논문

目的：应用中空纤维测定法评价藤甲酰苷（ garcinia glycosides ，GG）对8种人癌细胞的体内生长的抑制作用，通过裸鼠体内异位移植瘤实验，验证中空纤维测定法在抗肿瘤药效学研究中的可靠性。方法采用中空纤维测定法，将载有肿瘤细胞的中空纤维管植入NOD/SCID小鼠背部皮下，给药结束后取出中空纤维管，应用二苯基溴化四氮唑蓝（ MTT）比色法检测GG对各种肿瘤细胞在体内的抑瘤率；选取HL-60及B16人癌细胞，异位移植于BALb/c裸鼠右下肢外侧皮下，以环磷酰胺（ CTX）为阳性对照，各组连续给药10 d，于给药结束24 h后解剖小鼠，取出瘤块，计算CTX及GG的抑瘤率。结果应用中空纤维测定法及裸鼠体内异位移植瘤法测得GG高剂量组8 mg/（kg· d）、中剂量组4 mg/（kg· d）能明显抑制HL-60及B16人癌细胞在裸鼠体内的生长，实验测得结果与溶剂对照组比较，有显著性差异（P＜0．01）。结论应用中空纤维测定法测定样品GG对肿瘤细胞的生长抑制作用，实验结果与裸鼠体内异位移植瘤实验结果基本一致。该模型节约成本、效率提高、实验结果准确可靠，为GG的后续研究提供了指导和可靠证据。
목적：응용중공섬유측정법평개등갑선감（ garcinia glycosides ，GG）대8충인암세포적체내생장적억제작용，통과라서체내이위이식류실험，험증중공섬유측정법재항종류약효학연구중적가고성。방법채용중공섬유측정법，장재유종류세포적중공섬유관식입NOD/SCID소서배부피하，급약결속후취출중공섬유관，응용이분기추화사담서람（ MTT）비색법검측GG대각충종류세포재체내적억류솔；선취HL-60급B16인암세포，이위이식우BALb/c라서우하지외측피하，이배린선알（ CTX）위양성대조，각조련속급약10 d，우급약결속24 h후해부소서，취출류괴，계산CTX급GG적억류솔。결과응용중공섬유측정법급라서체내이위이식류법측득GG고제량조8 mg/（kg· d）、중제량조4 mg/（kg· d）능명현억제HL-60급B16인암세포재라서체내적생장，실험측득결과여용제대조조비교，유현저성차이（P＜0．01）。결론응용중공섬유측정법측정양품GG대종류세포적생장억제작용，실험결과여라서체내이위이식류실험결과기본일치。해모형절약성본、효솔제고、실험결과준학가고，위GG적후속연구제공료지도화가고증거。
Objective To evaluated the inhibitory effect of garcinia glycosides on growth of 8 kinds of human tumor cells in vi-vo by hollow fiber assay and confirm the reliability of hollow fiber assay in anticancer effect by the nude mice xenograft test .Methods Hollow fibers containing tumor cells were inserted underneath the skin of the NOD /SCID mice.The fibers were collected from the mice on the day after the administration and subjected to the stable endpoint MTT assay .The tumor cells of HL-60 and B16 were subcutane-ously implanted into the right flank of BALb /c nude mice.The positive control group was treated with cyclophosphamide .Each group was administered for 10 days.24 hours after the last administration , the mice were sacrificed and the tumors were excised and weigh-ted, the inhibition rate of tumor growth was calculated .Results The high-dose group of 8 mg/( kg· d) , middle dose group of 4 mg/( kg· d) of garcinia glycosides were measured by hollow fiber assay and nude mice test significantly inhibited the in vivo growth of HL -60 and B16 comparing with those in the solvent control group (P<0.01).Conclusion As a new model by hollow fiber assay to evalu-ate the inhibitory effect of garcinia glycosides , the test results were basically the same with nude mice test results .It made the experi-ment more rapidly , accurately and economically .An instruction and reliable evidence for follow-up study of garcinia glycosides was provided in this study .