生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2014年
4期
642-648
,共7页
栅藻%雨生红球藻%椭圆小球藻%生长特性%混合培养%城市二级出水
柵藻%雨生紅毬藻%橢圓小毬藻%生長特性%混閤培養%城市二級齣水
책조%우생홍구조%타원소구조%생장특성%혼합배양%성시이급출수
Scenedesmussp.%Chlorella ellipsoidea%Haematococcus pluvislis%growth characteristic%mixed culture%domestic secondary effluent
利用城市污水培养能源微藻可以实现水质净化和生物质生产的耦合，备受关注。生物质生产效率较低是限制其大规模应用的主要因素之一，混合培养是提高微藻生物质产率的一种潜在方法。为筛选出城市二级出水条件下合适的能源微藻混合藻种，考察了二级出水条件下3株高含油脂藻种栅藻LX1（Scenedesmussp. LX1）、椭圆小球藻YJ1（Chlorella ellipsoidea YJ1）和雨生红球藻（Haematococcus pluvislis）单一藻种和两两混合培养时的生长特性，比较了各微藻单一藻种及两两混合培养时的生长特性参数及生物质产量。研究结果表明，3种微藻的两两混合组合均能在二级出水条件下正常生长，各微藻干物质量浓度在第10天左右均能达到100 mg·L-1左右。与栅藻LX1和椭圆小球藻YJ1相比，雨生红球藻在两两混合培养条件下表现出更高的藻细胞干物质量浓度增长趋势。3种微藻的内禀生长速率均显著高于各自的单一培养，栅藻 LX1和雨生红球藻混合培养时分别达到最高内禀增长速率（分别为1.36 d-1、0.97 d-1）。栅藻LX1与雨生红球藻混合培养时比生长速率分别为0.59 d-1和0.42 d-1，分别比栅藻LX1和雨生红球藻的单一藻种培养提高了36%、9.0%。与单一藻种相比，混合培养促进了微藻的生物质产量，栅藻LX1和雨生红球藻混合藻种的生物质产量（277 mg·L-1）分别比栅藻LX1、雨生红球藻的单一藻种培养提高了64%和42%。栅藻LX1与雨生红球藻藻种组合具备作为二级出水条件下能源微藻培养合适混合藻种的潜力。
利用城市汙水培養能源微藻可以實現水質淨化和生物質生產的耦閤，備受關註。生物質生產效率較低是限製其大規模應用的主要因素之一，混閤培養是提高微藻生物質產率的一種潛在方法。為篩選齣城市二級齣水條件下閤適的能源微藻混閤藻種，攷察瞭二級齣水條件下3株高含油脂藻種柵藻LX1（Scenedesmussp. LX1）、橢圓小毬藻YJ1（Chlorella ellipsoidea YJ1）和雨生紅毬藻（Haematococcus pluvislis）單一藻種和兩兩混閤培養時的生長特性，比較瞭各微藻單一藻種及兩兩混閤培養時的生長特性參數及生物質產量。研究結果錶明，3種微藻的兩兩混閤組閤均能在二級齣水條件下正常生長，各微藻榦物質量濃度在第10天左右均能達到100 mg·L-1左右。與柵藻LX1和橢圓小毬藻YJ1相比，雨生紅毬藻在兩兩混閤培養條件下錶現齣更高的藻細胞榦物質量濃度增長趨勢。3種微藻的內稟生長速率均顯著高于各自的單一培養，柵藻 LX1和雨生紅毬藻混閤培養時分彆達到最高內稟增長速率（分彆為1.36 d-1、0.97 d-1）。柵藻LX1與雨生紅毬藻混閤培養時比生長速率分彆為0.59 d-1和0.42 d-1，分彆比柵藻LX1和雨生紅毬藻的單一藻種培養提高瞭36%、9.0%。與單一藻種相比，混閤培養促進瞭微藻的生物質產量，柵藻LX1和雨生紅毬藻混閤藻種的生物質產量（277 mg·L-1）分彆比柵藻LX1、雨生紅毬藻的單一藻種培養提高瞭64%和42%。柵藻LX1與雨生紅毬藻藻種組閤具備作為二級齣水條件下能源微藻培養閤適混閤藻種的潛力。
이용성시오수배양능원미조가이실현수질정화화생물질생산적우합，비수관주。생물질생산효솔교저시한제기대규모응용적주요인소지일，혼합배양시제고미조생물질산솔적일충잠재방법。위사선출성시이급출수조건하합괄적능원미조혼합조충，고찰료이급출수조건하3주고함유지조충책조LX1（Scenedesmussp. LX1）、타원소구조YJ1（Chlorella ellipsoidea YJ1）화우생홍구조（Haematococcus pluvislis）단일조충화량량혼합배양시적생장특성，비교료각미조단일조충급량량혼합배양시적생장특성삼수급생물질산량。연구결과표명，3충미조적량량혼합조합균능재이급출수조건하정상생장，각미조간물질량농도재제10천좌우균능체도100 mg·L-1좌우。여책조LX1화타원소구조YJ1상비，우생홍구조재량량혼합배양조건하표현출경고적조세포간물질량농도증장추세。3충미조적내품생장속솔균현저고우각자적단일배양，책조 LX1화우생홍구조혼합배양시분별체도최고내품증장속솔（분별위1.36 d-1、0.97 d-1）。책조LX1여우생홍구조혼합배양시비생장속솔분별위0.59 d-1화0.42 d-1，분별비책조LX1화우생홍구조적단일조충배양제고료36%、9.0%。여단일조충상비，혼합배양촉진료미조적생물질산량，책조LX1화우생홍구조혼합조충적생물질산량（277 mg·L-1）분별비책조LX1、우생홍구조적단일조충배양제고료64%화42%。책조LX1여우생홍구조조충조합구비작위이급출수조건하능원미조배양합괄혼합조충적잠력。
Energy microalgae cultivation in wastewater system is very promising for the combination of water purification and energy production. The coupled technology is limited by low biomass productivity of microalgae in wastewater. Mixed culture of specific energy microalgae is a potential solution to address the problem. In order to find out the proper mixed microalgae strains, Scenedesmussp. LX1,Chlorella ellipsoidea YJ1 andHaematococcus pluvisliswere chosen to study the growth characteristic among different microalgal species combinations under secondary effluent of domestic wastewater. Comparisons of the growth characteristic and biomass productivity were conducted among different microalgal species combinations. The results indicated that different microalgal species combination ofScenedesmus sp. LX1,Chlorella ellipsoideaYJ1 and Haematococcus pluvialis can grow well, with biomass of every microalgae growing up to 100 mg·L-1 on day 10. Compared withScenedesmus sp. LX1 andChlorella ellipsoidea YJ1,Haematococcus pluvislis exhibited higher biomass growth potential. The intrinsic growth rate of the three microalgae in the mixed culture was higher than the mono-culture of them. The highest intrinsic growth rate ofScenedesmus sp. LX1 andHaematococcus pluvislis (1.36 d-1, 0.97 d-1, respectively) was obtained under the mixed culture of them.Scenedesmussp. LX1 andHaematococcus pluvislisin the mixed culture of them behaved higher specific growth rate (0.59 d-1, 0.42 d-1, respectively) than each mono-culture of them. Compared with the mono-culture of Scenedesmus sp. LX1,Chlorella ellipsoideaYJ1 and Haematococcus pluvialis, the biomass productivity was enhanced in the mixed cultures. The biomass productivity in the mixed culture ofScenedesmus sp. LX1 andHaematococcus pluvialis was increased by 64% and 42% than the monoculture ofScenedesmus sp. LX1 andHaematococcus pluvialis, respectively. The mixed culture ofScenedesmus sp. LX1 andHaematococcus pluvialis are potential strains used in energy microalgae cultivation in secondary effluent of domestic wastewater.
