CAJ | 학술논문

以千日红种子为外植体，获得无菌苗，并采用正交试验设计方法进行无菌苗的增殖、生根壮苗培养和试管开花诱导．无菌苗最佳增殖培养基为： MS ＋1.0 mg?L -1 BA ＋0.5 mg?L -1 NAA ，培养28 d ，增殖系数为3.1，苗高可达3.3 cm ；最佳生根培养基为：1/2 MS ＋0.5 mg?L -1 IBA ＋30 g?L -1蔗糖＋0.5 g?L -1活性炭，并以透气膜为封口材料；无菌苗接种于改良 MS ＋5.0 mg?L -1 PP333＋40 g?L -1蔗糖＋7 g?L -1亚精胺，平均开花率达11.11％．采用组织培养方法获得无菌苗，并诱导其在试管内开花，可为其优良品种的快速繁殖和开发利用提供技术支持．
이천일홍충자위외식체，획득무균묘，병채용정교시험설계방법진행무균묘적증식、생근장묘배양화시관개화유도．무균묘최가증식배양기위： MS ＋1.0 mg?L -1 BA ＋0.5 mg?L -1 NAA ，배양28 d ，증식계수위3.1，묘고가체3.3 cm ；최가생근배양기위：1/2 MS ＋0.5 mg?L -1 IBA ＋30 g?L -1자당＋0.5 g?L -1활성탄，병이투기막위봉구재료；무균묘접충우개량 MS ＋5.0 mg?L -1 PP333＋40 g?L -1자당＋7 g?L -1아정알，평균개화솔체11.11％．채용조직배양방법획득무균묘，병유도기재시관내개화，가위기우량품충적쾌속번식화개발이용제공기술지지．
Seeds of Gomphrena globosa are acted as experimental materials to get aseptic seedling and then used for multiplication , rooting and flowering in vitro by orthogonal design . Results show that the optimal multiplication medium is : MS + 1.0 mg?L - 1 BA + 0.5 mg?L - 1 NAA , after 28 days of culture , the multiplication coefficient is 3.1 and the average seedling height reach to 3.3 cm . The optimized medium for rooting is 1/2 MS + 0.5 mg?L - 1 IBA + 30 g?L- 1 sucrose + 0.5 g?L- 1 activate charcoal with breathable film as sealing materials . The medium for flowering is modified MS + 5 mg?L - 1 PP333 + 40 g?L- 1 sucrose + 7 mg ? L- 1 spermidine ,with the average flowering rate is 11.11% . Aseptic seedling is obtained by tissue culture and then induced in vitro flowering , which is useful to provide technical support for the development and utilization of Gomphrena globosa by rapid propagation .