CAJ | 학술논문

采用恒电位沉积法，制备以L？酪氨酸为模板分子，壳聚糖为功能基体的分子印迹电化学传感器．实验结果表明：在优化的制备和测试条件下，所制得的印迹传感器对L？酪氨酸具有良好的特异识别性能，印迹因子达3．34；在 pH值为6．0，0．1 mol·L-1的磷酸盐缓冲溶液中，L？酪氨酸的微分脉冲伏安氧化峰峰电流与浓度在4．0×10-7～1．0×10-4 mol·L-1范围内呈良好的线性关系，检出限为2．0×10-7 mol·L-1．将该传感器用于人血清中酪氨酸含量的测定，平均回收率为89．50％～99．67％．
채용항전위침적법，제비이L？락안산위모판분자，각취당위공능기체적분자인적전화학전감기．실험결과표명：재우화적제비화측시조건하，소제득적인적전감기대L？락안산구유량호적특이식별성능，인적인자체3．34；재 pH치위6．0，0．1 mol·L-1적린산염완충용액중，L？락안산적미분맥충복안양화봉봉전류여농도재4．0×10-7～1．0×10-4 mol·L-1범위내정량호적선성관계，검출한위2．0×10-7 mol·L-1．장해전감기용우인혈청중락안산함량적측정，평균회수솔위89．50％～99．67％．
A novel molecularly imprinted electrochemical sensor was prepared with chitosan as functional matrix and L-tyrosine (L-Tyr)as template molecule by potentiostatic electrodeposition.According to our experimental results,the mo-lecular imprinted polymer (MIP)sensor with an imprinted factor of 3.34 showed well sensitivity and good selectivity to L-Tyr among the structural similarities and co-existences.The oxidation peak currents of differential pulse voltammetry were linear to the concentration of L-Tyr in the range from 4.0×10-7 to 1.0×10-4 mol·L-1 with a detection limit of 2. 0×10-7 mol·L-1 .Furthermore,the recoveries ranged from 89.50% to 99.67% were obtained when the sensor was ap-plied to real samples analysis.