暨南大学学报（自然科学与医学版）
暨南大學學報（自然科學與醫學版）
기남대학학보（자연과학여의학판）
JOURNAL OF JINAN UNIVERSITY（NATURAL SCIENCE & MEDICINE EDITION）
2014年
4期
381-390
,共10页
膜性肾病%小核糖核酸%靶基因%GO富集%KEGG通路
膜性腎病%小覈糖覈痠%靶基因%GO富集%KEGG通路
막성신병%소핵당핵산%파기인%GO부집%KEGG통로
membranous nephropathy%microRNA%target gene%GO enrichment%KEGG pathways
目的：探讨膜性肾病（MN）差异性表达microRNA的靶基因主要参与的Gene Ontology（GO）富集与Kyo-to Encyclopedia Genes And Genomes（KEGG）通路．方法：100例MN患者与100例健康对照者（NC）作为实验对象．100例NC随机分成10组（NC1～10），每组10人．同样100例MN随机分成10组，每组10人（MN1～10）．分别从实验参与者静脉采取全血10 mL，来源于静脉血的单个核细胞用于提取RNA．总RNA以组为单位等量混合用于高通量技术测序（high-throughput sequencing），对RNA测序结果进行长度分布，基因比对，RNA分类注释，RNA特有序列及其公共序列分析后，获得小核糖核酸（microRNA）进行MN与NC差异性表达分析来寻找显著表达microRNA．应用相关的软件对差异性表达的microRNA进行靶基因预测，靶基因借助功能注释软件对其参与的生物过程进行GO富集及KE GG通路分析．结果：靶基因在GO富集分析中主要集中在细胞器官组成，细胞膜组成，离子结合，生物代谢过程，生物调节过程等．靶基因在KE GG通路分析中主要参与嘌呤代谢通路，代谢产物生物合成，癌症通路，蛋白激酶信号通路等．结论：MN与NC之间存在着显著差异性表达microRNA．差异性表达的microRNA的靶基因参与的GO富集与KE GG通路可能与MN的发病机制与临床症状有着密切的联系．
目的：探討膜性腎病（MN）差異性錶達microRNA的靶基因主要參與的Gene Ontology（GO）富集與Kyo-to Encyclopedia Genes And Genomes（KEGG）通路．方法：100例MN患者與100例健康對照者（NC）作為實驗對象．100例NC隨機分成10組（NC1～10），每組10人．同樣100例MN隨機分成10組，每組10人（MN1～10）．分彆從實驗參與者靜脈採取全血10 mL，來源于靜脈血的單箇覈細胞用于提取RNA．總RNA以組為單位等量混閤用于高通量技術測序（high-throughput sequencing），對RNA測序結果進行長度分佈，基因比對，RNA分類註釋，RNA特有序列及其公共序列分析後，穫得小覈糖覈痠（microRNA）進行MN與NC差異性錶達分析來尋找顯著錶達microRNA．應用相關的軟件對差異性錶達的microRNA進行靶基因預測，靶基因藉助功能註釋軟件對其參與的生物過程進行GO富集及KE GG通路分析．結果：靶基因在GO富集分析中主要集中在細胞器官組成，細胞膜組成，離子結閤，生物代謝過程，生物調節過程等．靶基因在KE GG通路分析中主要參與嘌呤代謝通路，代謝產物生物閤成，癌癥通路，蛋白激酶信號通路等．結論：MN與NC之間存在著顯著差異性錶達microRNA．差異性錶達的microRNA的靶基因參與的GO富集與KE GG通路可能與MN的髮病機製與臨床癥狀有著密切的聯繫．
목적：탐토막성신병（MN）차이성표체microRNA적파기인주요삼여적Gene Ontology（GO）부집여Kyo-to Encyclopedia Genes And Genomes（KEGG）통로．방법：100례MN환자여100례건강대조자（NC）작위실험대상．100례NC수궤분성10조（NC1～10），매조10인．동양100례MN수궤분성10조，매조10인（MN1～10）．분별종실험삼여자정맥채취전혈10 mL，래원우정맥혈적단개핵세포용우제취RNA．총RNA이조위단위등량혼합용우고통량기술측서（high-throughput sequencing），대RNA측서결과진행장도분포，기인비대，RNA분류주석，RNA특유서렬급기공공서렬분석후，획득소핵당핵산（microRNA）진행MN여NC차이성표체분석래심조현저표체microRNA．응용상관적연건대차이성표체적microRNA진행파기인예측，파기인차조공능주석연건대기삼여적생물과정진행GO부집급KE GG통로분석．결과：파기인재GO부집분석중주요집중재세포기관조성，세포막조성，리자결합，생물대사과정，생물조절과정등．파기인재KE GG통로분석중주요삼여표령대사통로，대사산물생물합성，암증통로，단백격매신호통로등．결론：MN여NC지간존재착현저차이성표체microRNA．차이성표체적microRNA적파기인삼여적GO부집여KE GG통로가능여MN적발병궤제여림상증상유착밀절적련계．
Aim:To explore the target gene which came from different expression microRNA in mem-branous nephropathy (MN)and mainly participated in the Gene Ontology (GO)enrichment and Kyoto Encyclopedia Of Genes And Genomes (KEGG)pathways.Methods:The participants in this research included 100 MN patients and 100 healthy controls (NC).One hundred healthy controls were divided in-to 10 groups,each group contained 10 persons.And 100 MN were also divided into 10 groups,each group contained 10 persons.Draw venous blood (10 mL)from each participant,respectively.Then the peripheral blood mononuclear cells were separated from venous blood to extract total RNA.Equal total RNA was compounded base on groups as unit was subjected to high-throughput sequencing.After this primary process,the RNA was used to preliminary analysis which included length distribution,genome mapping,RNA annotation,common and specific sequences accounted.The microRNA finally was ob-tained.We made a comparsion of microRNAs between MN and NC groups to find the different expression microRNA.The different expression microRNAs were used to predict target microRNA through relevant software.The target genes came from different expression microRNAs were subjected to GO enrichment and KEGG pathways analysis.Results:The target genes mainly enriched in cellular part,cellular mem-brance,ion binding,biopolymer metabolic process,regulation of biological process.For the KEGG path-way,the target gene mainly participated in purine metabolism,biosynthesis of secondary metabolites, pathway in cancer,MAPK signaling pathway.Conclusion:There were different expression microRNAs between MN and NC1 ~10 groups.The target gene came from different expression microRNAs that in the GO enrichment and KEGG pathway analysis could have the relation with the pathogenesis and clinical symptom of MN.
