暨南大学学报（自然科学与医学版）
暨南大學學報（自然科學與醫學版）
기남대학학보（자연과학여의학판）
JOURNAL OF JINAN UNIVERSITY（NATURAL SCIENCE & MEDICINE EDITION）
2014年
4期
372-376
,共5页
陈顺萍%张荣莲%任坤海%王梅颖
陳順萍%張榮蓮%任坤海%王梅穎
진순평%장영련%임곤해%왕매영
精液%乙型肝炎病毒%父婴%垂直传播
精液%乙型肝炎病毒%父嬰%垂直傳播
정액%을형간염병독%부영%수직전파
semen%hepatitis B virus%father to infant%vertical transmission
目的：探讨乙肝表面抗原（HBsAg）阳性父亲精液乙型肝炎病毒脱氧核糖核酸（HBV-DNA）载量对其新生儿发生HBV父婴垂直传播的影响，以期寻找阻断HBV父婴垂直传播的有效方法．方法：在知情同意的原则，以丈夫血清HBsAg阳性、孕母HBsAg及HBV-DNA均阴性的52个家庭作为研究对象，收集父母及其新生儿的相关资料及血液标本和父亲精液标本，检测血液乙肝病毒血清学标志物（HBVM）、HBV-DNA载量与精液HBV-DNA载量．依据其新生儿脐带血HBV-DNA检测结果作为分组标准，HBV-DNA检测为阳性11例为病例组，阴性41例为对照组，进行病例对照研究．结果：新生儿脐带血 HBV-DNA 阳性率为21.2％（11/52），52份精液 HBV-DNA 阳性率为26.9％（14/52）；精液HBV-DNA阳性父亲其新生儿发生HBV父婴垂直传播的危险性高于精液HBV-DNA阴性者（P＜0.01）；精液与其血清的HBV-DNA载量存在正相关关系，精液HBV-DNA载量低于其血清载量；ROC曲线分析显示，精液HBV-DNA载量在预测HBV垂直传播发生风险的效果优于血清HBV-DNA载量．结论：父亲精液HBV-DNA载量是HBV父婴垂直传播的危险因素；HBsAg阳性的男性在计划孕育前降低其血液及精液中HBV-DNA载量水平能降低HBV父婴垂直传播的风险．
目的：探討乙肝錶麵抗原（HBsAg）暘性父親精液乙型肝炎病毒脫氧覈糖覈痠（HBV-DNA）載量對其新生兒髮生HBV父嬰垂直傳播的影響，以期尋找阻斷HBV父嬰垂直傳播的有效方法．方法：在知情同意的原則，以丈伕血清HBsAg暘性、孕母HBsAg及HBV-DNA均陰性的52箇傢庭作為研究對象，收集父母及其新生兒的相關資料及血液標本和父親精液標本，檢測血液乙肝病毒血清學標誌物（HBVM）、HBV-DNA載量與精液HBV-DNA載量．依據其新生兒臍帶血HBV-DNA檢測結果作為分組標準，HBV-DNA檢測為暘性11例為病例組，陰性41例為對照組，進行病例對照研究．結果：新生兒臍帶血 HBV-DNA 暘性率為21.2％（11/52），52份精液 HBV-DNA 暘性率為26.9％（14/52）；精液HBV-DNA暘性父親其新生兒髮生HBV父嬰垂直傳播的危險性高于精液HBV-DNA陰性者（P＜0.01）；精液與其血清的HBV-DNA載量存在正相關關繫，精液HBV-DNA載量低于其血清載量；ROC麯線分析顯示，精液HBV-DNA載量在預測HBV垂直傳播髮生風險的效果優于血清HBV-DNA載量．結論：父親精液HBV-DNA載量是HBV父嬰垂直傳播的危險因素；HBsAg暘性的男性在計劃孕育前降低其血液及精液中HBV-DNA載量水平能降低HBV父嬰垂直傳播的風險．
목적：탐토을간표면항원（HBsAg）양성부친정액을형간염병독탈양핵당핵산（HBV-DNA）재량대기신생인발생HBV부영수직전파적영향，이기심조조단HBV부영수직전파적유효방법．방법：재지정동의적원칙，이장부혈청HBsAg양성、잉모HBsAg급HBV-DNA균음성적52개가정작위연구대상，수집부모급기신생인적상관자료급혈액표본화부친정액표본，검측혈액을간병독혈청학표지물（HBVM）、HBV-DNA재량여정액HBV-DNA재량．의거기신생인제대혈HBV-DNA검측결과작위분조표준，HBV-DNA검측위양성11례위병례조，음성41례위대조조，진행병례대조연구．결과：신생인제대혈 HBV-DNA 양성솔위21.2％（11/52），52빈정액 HBV-DNA 양성솔위26.9％（14/52）；정액HBV-DNA양성부친기신생인발생HBV부영수직전파적위험성고우정액HBV-DNA음성자（P＜0.01）；정액여기혈청적HBV-DNA재량존재정상관관계，정액HBV-DNA재량저우기혈청재량；ROC곡선분석현시，정액HBV-DNA재량재예측HBV수직전파발생풍험적효과우우혈청HBV-DNA재량．결론：부친정액HBV-DNA재량시HBV부영수직전파적위험인소；HBsAg양성적남성재계화잉육전강저기혈액급정액중HBV-DNA재량수평능강저HBV부영수직전파적풍험．
Aim:To explore the impact of the load levels of paternal semen HBV-DNA on vertical transmission of HBV from HBsAg-positive father to infant.Methods:52 families of pregnant women with negative HBsAg and HBV-DNA and husbands with positive,serum HBsAg were selected.Clinical data and blood samples of the parents and their newborns、semen samples of the husbands were collected.Ser-um HBVM and the load levels of paternal blood and semen HBV-DNA were determined.In case-control study,based on the results of neonatal cord blood HBV-DNA detection,1 1 newborns with cord blood posi-tive HBV-DNA were selected as subiects,and 41 newborns with negative HBV-DNA as controls.Results:①The positive rate of neonatal cord blood HBV-DNA was found to be 21.2%(11/52),and that of se-men HBV-DNA was 26.9%(14/52).②The incidence of vertical transmission of HBV in infants with paternal positive semen HBV-DNA was found significantly higher than that in infants with paternal nega-tive semen HBV-DNA(P<0.01).③There was a positive rank correlation between semen and serum of HBV-DNA load levels,while the load levels of semen HBV-DNA was lower than that of serum HBV-DNA load levels.④The analysis of ROC curve showed that the prediction accuracy of semen HBV-DNA in the occurrence of vertical transmission were more accurate than that based on serum HBV-DNA load.Con-clusion:Paternal positive semen HBV-DNA is one of the risk fators for vertical transmission of HBV;re-ducing HBV-DNA load levels in paternal blood and semen before pregnancy may be a way to block father-fetal transmisson of HBV.
