CAJ | 학술논문

采用正交试验L9（34），对加热回流提取和超声提取优化广东紫珠总黄酮的提取工艺进行了比较研究.以提取物中总黄酮得率为评价指标，利用紫外分光光度法测定黄酮的含量.结果表明，加热回流提取的最佳工艺条件为A1 B3 C2，即乙醇浓度为60％、水浴温度为80℃、提取时间为2 h；超声法为A2 B1 C2，即乙醇浓度为70％、固液比为1：20、超声40 min.芦丁对照品9.4～75.2μg?mL-1范围内与吸光度呈良好的线性关系.加热回流提取法优于超声法，黄酮提取率达到9.27％.
채용정교시험L9（34），대가열회류제취화초성제취우화엄동자주총황동적제취공예진행료비교연구.이제취물중총황동득솔위평개지표，이용자외분광광도법측정황동적함량.결과표명，가열회류제취적최가공예조건위A1 B3 C2，즉을순농도위60％、수욕온도위80℃、제취시간위2 h；초성법위A2 B1 C2，즉을순농도위70％、고액비위1：20、초성40 min.호정대조품9.4～75.2μg?mL-1범위내여흡광도정량호적선성관계.가열회류제취법우우초성법，황동제취솔체도9.27％.
To optimize the extracting process of the total flavonoids from Callicarpa kwangtungensis . The extract technology of the total flavonoids from arpa kwangtungensis was optimized by orthogonal experiment L9 (33 ) using reflux extraction and ultrasonic extraction with the yield of total flavonoids as index . The optimum extracting conditions of reflux extraction and ultrasonic extraction for the total flavonoids from Callicarpa kwangtungensis were as follows respective :the refux extraction was conducted 3 hour at 80℃ with 60% ethanol as solvent and ultrasounded 40 minutes by the ratio of solid to solution of 1:20 with 70% ethanol as solvent . The linear range of Rutin was 9.4-75.2 μg . mL-1 . The reflux extraction is better than ultrasonic extraction , and the optimum extracting technology is simple and reasonable , and it serves as good reference for the extracting process of the total flavonoids from Callicarpa kwangtungensis .