中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
18期
38-40,43
,共4页
胰岛素样生长因子-1%瘦素%生长激素缺乏症%特发性矮小
胰島素樣生長因子-1%瘦素%生長激素缺乏癥%特髮性矮小
이도소양생장인자-1%수소%생장격소결핍증%특발성왜소
Insulin-like growth factor-1%Leptin%Growth hormone deficiency%Idiopathic short stature
目的：了解不同病因的矮小儿童血清胰岛素样生长因子-员渊IGF-1冤和瘦素水平变化袁探讨IGF-1和瘦素在生长激素缺乏症渊GHD冤诊断中的价值遥方法选择深圳市妇幼保健院就诊的矮小儿童96例袁年龄5~11岁袁根据身高低于同性别同年龄均值-2~-3SD袁排除其他可致身材矮小疾病袁根据生长激素激发试验袁GH峰值约10 ng/mL为GHD者共67例渊GHD组冤尧GH峰值逸10 ng/mL为特发性矮小渊ISS冤者共29例渊ISS组冤袁另选择同期体检的生长发育正常儿童23名作为对照组遥采集所有研究对象的外周静脉血3 mL袁分离血清后袁使用双抗体一步夹心法酶联免疫吸附实验渊ELISA冤测定血清IGF-1尧瘦素浓度遥结果 GHD组血清IGF-1及瘦素水平[渊45.7依19.02冤滋g/L尧渊4.25依0.63冤mg/L]低于ISS组[渊114.07依24.45冤滋g/L尧(4.69依0.69) mg/L]和对照组[渊164.61依46.22冤滋g/L尧(6.27依0.89) mg/L]冤袁组间比较袁差异均有高度统计学意义渊P<0.01冤曰诊断GHD袁IGF-1的敏感度为100%袁特异度为98%曰瘦素的敏感度为88%袁特异度为71%遥结论 IGF-1和瘦素的检测可能可作为筛查和诊断GHD有价值的指标袁并对GHD与ISS的鉴别诊断也有着一定的临床意义。
目的：瞭解不同病因的矮小兒童血清胰島素樣生長因子-員淵IGF-1冤和瘦素水平變化袁探討IGF-1和瘦素在生長激素缺乏癥淵GHD冤診斷中的價值遙方法選擇深圳市婦幼保健院就診的矮小兒童96例袁年齡5~11歲袁根據身高低于同性彆同年齡均值-2~-3SD袁排除其他可緻身材矮小疾病袁根據生長激素激髮試驗袁GH峰值約10 ng/mL為GHD者共67例淵GHD組冤堯GH峰值逸10 ng/mL為特髮性矮小淵ISS冤者共29例淵ISS組冤袁另選擇同期體檢的生長髮育正常兒童23名作為對照組遙採集所有研究對象的外週靜脈血3 mL袁分離血清後袁使用雙抗體一步夾心法酶聯免疫吸附實驗淵ELISA冤測定血清IGF-1堯瘦素濃度遙結果 GHD組血清IGF-1及瘦素水平[淵45.7依19.02冤滋g/L堯淵4.25依0.63冤mg/L]低于ISS組[淵114.07依24.45冤滋g/L堯(4.69依0.69) mg/L]和對照組[淵164.61依46.22冤滋g/L堯(6.27依0.89) mg/L]冤袁組間比較袁差異均有高度統計學意義淵P<0.01冤曰診斷GHD袁IGF-1的敏感度為100%袁特異度為98%曰瘦素的敏感度為88%袁特異度為71%遙結論 IGF-1和瘦素的檢測可能可作為篩查和診斷GHD有價值的指標袁併對GHD與ISS的鑒彆診斷也有著一定的臨床意義。
목적：료해불동병인적왜소인동혈청이도소양생장인자-원연IGF-1원화수소수평변화원탐토IGF-1화수소재생장격소결핍증연GHD원진단중적개치요방법선택심수시부유보건원취진적왜소인동96례원년령5~11세원근거신고저우동성별동년령균치-2~-3SD원배제기타가치신재왜소질병원근거생장격소격발시험원GH봉치약10 ng/mL위GHD자공67례연GHD조원요GH봉치일10 ng/mL위특발성왜소연ISS원자공29례연ISS조원원령선택동기체검적생장발육정상인동23명작위대조조요채집소유연구대상적외주정맥혈3 mL원분리혈청후원사용쌍항체일보협심법매련면역흡부실험연ELISA원측정혈청IGF-1요수소농도요결과 GHD조혈청IGF-1급수소수평[연45.7의19.02원자g/L요연4.25의0.63원mg/L]저우ISS조[연114.07의24.45원자g/L요(4.69의0.69) mg/L]화대조조[연164.61의46.22원자g/L요(6.27의0.89) mg/L]원원조간비교원차이균유고도통계학의의연P<0.01원왈진단GHD원IGF-1적민감도위100%원특이도위98%왈수소적민감도위88%원특이도위71%요결론 IGF-1화수소적검측가능가작위사사화진단GHD유개치적지표원병대GHD여ISS적감별진단야유착일정적림상의의。
Objective To understand the levels of serum insulin-like growth factor-1 (IGF-1) and leptin and to discuss the value of IGF-1 and leptin in the diagnosis of growth hormone deficiency (GHD). Methods A total of 96 patients were recruited in Shenzhen Maternity and Child Healthcare Hospital, whose height lower than -2--3SD of the same age and the same sex mean height, to rule out other diseases can cause short stature, according to growth hormone stim-ulation test, children with GHD whose GH peak<10 ng/mL were as GHD group (n=67), children with idiopathic short stature (ISS) whose GH peak≥10 ng/mL were as ISS group (n=29). The age of patients were at a range of 5-11 years, 23 children aged from 5 to 11 years old whose physical development were natural were chosen as control group. 3 mL peripheral blood of all subjects was chosen as the sample. The concentration of serum IGF-1 and leptin was measured by double antibody step clip art enzyme-linked immunosorbent assay (ELISA) method. Results The levels of serum IGF-1 and leptin in GHD group [(45.7±19.02) μg/L, (4.25±0.63) mg/L] were significantly lower than those of ISS group [(114.07±24.45) μg/L, (4.69±0.69) mg/L], and control group [(164.61±46.22) μg/L, (6.27±0.89) mg/L], the differences were highly statistically significant (P< 0.01). The diagnostic sensitivity of the IGF-1 was 100%, and the specificity was 98%;the diagnostic sensitivity of the leptin was 88%, and specificity was 71%. Conclusion The detection of IGF-1 and leptin can be used as the screening and diagnosis of GHD valuable indicators, and it also has a certain clinical significance to the differential diagnosis of GHD and the ISS.
