CAJ | 학술논문

目的：探讨人类免疫缺陷病毒玉型(HIV-1)B忆亚型感染者人类白细胞抗原(HLA)-A、-B、-C位点等位基因的分布及其对病毒载量的影响.方法对146例HIV-1B忆亚型感染者采用聚合酶链反应-序列特异性引物(PCR-SSP)扩增方法进行HLA-A、-B、-C位点等位基因检测,计算各位点等位基因的频率及其对感染者病毒载量的影响.结果在146例HIV-1B忆亚型感染者中分别检出HLA-A位点14个等位基因,-B位点24个等位基因,-Cw位点12个等位基因,其中频率大于0.1的等位基因为HLA-A*02,-A*11,-A*24,-A*30,-B*13,-B*40,-B*51,-Cw*03,-Cw*06,-Cw*08；HLA-A、-B、-C等位基因纯合子携带者的病毒载量高于杂合子(P=0.0013)；HLA-A*03(P=0.0314)、-A*30(P=0.0072)、-B*13(P=0.0087)、-Cw*06(P=0.0145)等位基因携带者具有较低病毒载量.结论 HIV-1B忆亚型感染者HLA-A*03、-A*30、-B*13、-Cw*06等位基因与低病毒载量相关。
목적：탐토인류면역결함병독옥형(HIV-1)B억아형감염자인류백세포항원(HLA)-A、-B、-C위점등위기인적분포급기대병독재량적영향.방법대146례HIV-1B억아형감염자채용취합매련반응-서렬특이성인물(PCR-SSP)확증방법진행HLA-A、-B、-C위점등위기인검측,계산각위점등위기인적빈솔급기대감염자병독재량적영향.결과재146례HIV-1B억아형감염자중분별검출HLA-A위점14개등위기인,-B위점24개등위기인,-Cw위점12개등위기인,기중빈솔대우0.1적등위기인위HLA-A*02,-A*11,-A*24,-A*30,-B*13,-B*40,-B*51,-Cw*03,-Cw*06,-Cw*08；HLA-A、-B、-C등위기인순합자휴대자적병독재량고우잡합자(P=0.0013)；HLA-A*03(P=0.0314)、-A*30(P=0.0072)、-B*13(P=0.0087)、-Cw*06(P=0.0145)등위기인휴대자구유교저병독재량.결론 HIV-1B억아형감염자HLA-A*03、-A*30、-B*13、-Cw*06등위기인여저병독재량상관。
Objective To study the distribution of HLA-A, -B, and C alleles in HIV-1 B’infected individuals and to analyze its impact on HIV-1 viral load. Methods 146 HIV-1 infected individuals were recruited and their HLA-A,-B, and C alleles were genotyped using PCR-SSP technique. Allele frequencies and association of HLA-A, -B, and C alleles with HIV-1 viral loads were also analyzed. Results 14 HLA-A allels, 24 HLA-B allels, and 12 HLA-Cw allels were detected in 146 HIV-1 B' infected individuals. Of them, HLA-A*02, -A*11, -A*24, -A*30, -B*13, -B*40,-B*51,-Cw*03,-Cw*06,-Cw*08 were among the most common alleles with frequencies above 0.1;subjects homozy-gous at HLA class Ⅰ loci had higher viral loads (P=0.0013), subjects carrying HLA-A*03 (P=0.0314), -A*30 (P=0.0072), -B*13 (P=0.0087) and -Cw*06 (P=0.0145) alleles had lower viral loads. Conclusion HLA-A*03, -A*30,-B*13 and-Cw*06 alleles may associate with lower viral loads in HIV-1 B’infected individuals.