CAJ | 학술논문

[目的]通过建立与番茄黄化曲叶病毒病抗病基因Ty-3紧密连锁的SCAR标记，以提高抗病材料选育效率与准确性。[方法]设计特异引物对相关材料进行PCR扩增，获得与抗病基因Ty-3连锁的SCAR分子标记。[结果]利用该标记检测抗病纯合材料（Ty-3/Ty-3）、感病材料（ty-3/ty-3）分别产生600 bp、320 bp片段，抗病杂合材料（Ty-3/ty-3）产生600 bp与320 bp 2条片段；利用该标记对7份田间表现抗病的番茄F1代杂交种进行检测，有4份含有抗病基因Ty-3，3份未检出；选择经济性状优良、含有抗病基因Ty-3的F1代杂交种自交得到F2群体28个单株，利用该标记对单株进行检测，得到抗病纯合单株7个，抗病杂合单株13个，感病单株8个。[结论]该标记是与抗病基因Ty-3紧密连锁的共显性标记，可用于番茄抗病基因Ty-3的鉴定与抗病材料的筛选。
[목적]통과건립여번가황화곡협병독병항병기인Ty-3긴밀련쇄적SCAR표기，이제고항병재료선육효솔여준학성。[방법]설계특이인물대상관재료진행PCR확증，획득여항병기인Ty-3련쇄적SCAR분자표기。[결과]이용해표기검측항병순합재료（Ty-3/Ty-3）、감병재료（ty-3/ty-3）분별산생600 bp、320 bp편단，항병잡합재료（Ty-3/ty-3）산생600 bp여320 bp 2조편단；이용해표기대7빈전간표현항병적번가F1대잡교충진행검측，유4빈함유항병기인Ty-3，3빈미검출；선택경제성상우량、함유항병기인Ty-3적F1대잡교충자교득도F2군체28개단주，이용해표기대단주진행검측，득도항병순합단주7개，항병잡합단주13개，감병단주8개。[결론]해표기시여항병기인Ty-3긴밀련쇄적공현성표기，가용우번가항병기인Ty-3적감정여항병재료적사선。
Objective The aim was to obtain SCAR marker tightly linked for resistance gene Ty-3 in tomato, which would increase the efficiency and accuracy of resistance materials. [Method] The specific primer was designed for PCR amplified. The SCAR molecular marker which was linked to Ty-3 was detected. [Result] With the SCAR markerm, resistant and susceptible homozygous materials produced about 600 bp and 320 bp PCR fragments respectively and resistant heterozygous produced 600/320 bp fragments. Ty-3 resistance gene of 7 F1 were detected with this marker, The results showed that 4 hybrids contained Ty-3, but 3 hybrids not. Though all of them showed resistance. The SCAR marker was also used to detect Ty-3 resistance gene of F2 individuals. The results showed that 7 individuals contained Ty-3/Ty-3, 13 individuals contained Ty-3/ty-3 . [Conclusion] The SCAR marker which could distinguish resistant ,susceptible and co-dominant marker tightly linked to Ty-3 gene and could be used for MAS.