化学分析计量
化學分析計量
화학분석계량
CHEMICAL ANALYSIS AND METERAGE
2014年
4期
48-50
,共3页
液相色谱-串联质谱法%二噻农%草莓
液相色譜-串聯質譜法%二噻農%草莓
액상색보-천련질보법%이새농%초매
liquid chromatography-tandem mass spectrometry%dithianon%strawberry
采用盐酸酸化乙腈提取草莓中二噻农残留，提取溶液经无水硫酸钠和PSA净化后，利用安捷伦Poroshell 120 EC–C18色谱柱进行分离，以甲醇–水(体积比20∶80，含5 mmol／L乙酸铵溶液)作为流动相，ESI负离子模式下进行液相色谱–四极杆串联质谱分析。结果表明，在10～200μg／L浓度范围内，线性关系良好，r2＞0.9998，平均回收率为71.8％～78.3%，相对标准偏差小于5%(n=6)，方法定量限为5μg／kg。该方法简单快速，可满足新鲜草莓中二噻农残留检测工作的需要。
採用鹽痠痠化乙腈提取草莓中二噻農殘留，提取溶液經無水硫痠鈉和PSA淨化後，利用安捷倫Poroshell 120 EC–C18色譜柱進行分離，以甲醇–水(體積比20∶80，含5 mmol／L乙痠銨溶液)作為流動相，ESI負離子模式下進行液相色譜–四極桿串聯質譜分析。結果錶明，在10～200μg／L濃度範圍內，線性關繫良好，r2＞0.9998，平均迴收率為71.8％～78.3%，相對標準偏差小于5%(n=6)，方法定量限為5μg／kg。該方法簡單快速，可滿足新鮮草莓中二噻農殘留檢測工作的需要。
채용염산산화을정제취초매중이새농잔류，제취용액경무수류산납화PSA정화후，이용안첩륜Poroshell 120 EC–C18색보주진행분리，이갑순–수(체적비20∶80，함5 mmol／L을산안용액)작위류동상，ESI부리자모식하진행액상색보–사겁간천련질보분석。결과표명，재10～200μg／L농도범위내，선성관계량호，r2＞0.9998，평균회수솔위71.8％～78.3%，상대표준편차소우5%(n=6)，방법정량한위5μg／kg。해방법간단쾌속，가만족신선초매중이새농잔류검측공작적수요。
Dithianon residue was extracted with acidic acetonitrile,the extract was purified by anhydrous sodium sulfate and PSA powder,and separated by Agilent Poroshell 120 EC–C18 column with methanol–water (volum ratio was 20∶80,containing 5 mmol/L ammonium acetate) as the mobile phase,and then determined by using liquid chromatography–tandem mass spectrometry under ESI negative ion mode. The linear relationship was fine in the range of 10–200 μg/L for dithianon, the relative coefficients r2<0.999 8. The recovery was 71.8%–78.3%with the relative standard deviations (RSDs) less than 5%(n=6),and the quantification limit was 5 μg/kg. The developed method is simple, fast and can meet the requirements of determining dithianon in fresh strawberry.
