山西科技
山西科技
산서과기
SHANXI KEJI
2014年
4期
79-81,82
,共4页
板蓝根泡腾片%浸膏%腺苷%(R,S)-告依春%高效液相色谱法%含量测定
闆藍根泡騰片%浸膏%腺苷%(R,S)-告依春%高效液相色譜法%含量測定
판람근포등편%침고%선감%(R,S)-고의춘%고효액상색보법%함량측정
effervescent tablet of Isatis indigotica fort%extract%adenosine%(R,S)-goitrin%HPLC%content determination
目的：建立板蓝根泡腾片浸膏中腺苷和（R，S）-告依春含量的高效液相色谱测定方法。方法：高效液相色谱仪Waters 2695-2487；Diamonsil C18柱（250 mm×4.6 mm，5μm）；柱温：30℃；流动相：甲醇-水（10∶90）；流速为1.0 mL/min；检测波长：255 nm。结果：精密度和稳定性均良好，腺苷的质量浓度在1.012μg/mL～30.36μg/mL范围内，线性关系良好，r＝1，平均回收率为96.7％，RSD为1.1％；（R，S）-告依春的质量浓度在2.042μg/mL～61.26μg/mL范围内，线性关系良好，r＝1，平均回收率为97.9％，RSD为0.7％。结论：该法简便快速，结果准确，重复性好，可作为板蓝根泡腾片浸膏的质量控制方法。
目的：建立闆藍根泡騰片浸膏中腺苷和（R，S）-告依春含量的高效液相色譜測定方法。方法：高效液相色譜儀Waters 2695-2487；Diamonsil C18柱（250 mm×4.6 mm，5μm）；柱溫：30℃；流動相：甲醇-水（10∶90）；流速為1.0 mL/min；檢測波長：255 nm。結果：精密度和穩定性均良好，腺苷的質量濃度在1.012μg/mL～30.36μg/mL範圍內，線性關繫良好，r＝1，平均迴收率為96.7％，RSD為1.1％；（R，S）-告依春的質量濃度在2.042μg/mL～61.26μg/mL範圍內，線性關繫良好，r＝1，平均迴收率為97.9％，RSD為0.7％。結論：該法簡便快速，結果準確，重複性好，可作為闆藍根泡騰片浸膏的質量控製方法。
목적：건립판람근포등편침고중선감화（R，S）-고의춘함량적고효액상색보측정방법。방법：고효액상색보의Waters 2695-2487；Diamonsil C18주（250 mm×4.6 mm，5μm）；주온：30℃；류동상：갑순-수（10∶90）；류속위1.0 mL/min；검측파장：255 nm。결과：정밀도화은정성균량호，선감적질량농도재1.012μg/mL～30.36μg/mL범위내，선성관계량호，r＝1，평균회수솔위96.7％，RSD위1.1％；（R，S）-고의춘적질량농도재2.042μg/mL～61.26μg/mL범위내，선성관계량호，r＝1，평균회수솔위97.9％，RSD위0.7％。결론：해법간편쾌속，결과준학，중복성호，가작위판람근포등편침고적질량공제방법。
Purpose : To establish a method for the determination of adenosine and (R,S)-goitrin of extract of effervescent tablet of Isatis indigotica fort by HPLC (high performance liquid chromatograph). Methods: The chromatographic conditions:HPLC Waters 2695-2487; Diamonsil C18 analytic chromatographic column (250 mm ×4.6 mm,5μm);column temperature:30℃;the mobile phase consisted of methanol-water (10:90);eluted at a rate of 1.0 mL/min;the wavelength for detection:255 nm. Results:The precision and stability were fine.The standard curves of adenosine was linear in the concentration range of 1.012μg/mL~30.36 μg/mL,r=1; the average recovery was 96.7%,with RSD of 1.1%. And the standard curves of (R,S)-goitrin was linear in the concentration range of 2.042μg/mL~61.26μg/mL,r=1;the average recovery was 97.9% with RSD of 0.7%. Conclusion: This method was sensitive,quick and accurate for the quality control of extractum of effervescent tablet of Isatis indigotica fort.
