CAJ | 학술논문

从患典型肠套叠症的濒死斑点叉尾（Ictalurus punctatus）肝脏分离到1株病原菌GX-1，经API 20NE鉴定为温和气单胞菌（Aeromonas sobria）。16S rRNA基因测序结果，该菌株与温和气单胞菌NCIMB 12065株的亲缘关系最近，相似性达99.8％。该菌株对健康斑点叉尾鱼种和成鱼均具有很强的致病力，人工感染发病鱼症状与自然病鱼症状基本一致。药敏试验结果表明， GX-1对头孢拉定、先锋必等15种抗生素敏感，对苯唑青霉素和青霉素G耐药。
종환전형장투첩증적빈사반점차미（Ictalurus punctatus）간장분리도1주병원균GX-1，경API 20NE감정위온화기단포균（Aeromonas sobria）。16S rRNA기인측서결과，해균주여온화기단포균NCIMB 12065주적친연관계최근，상사성체99.8％。해균주대건강반점차미어충화성어균구유흔강적치병력，인공감염발병어증상여자연병어증상기본일치。약민시험결과표명， GX-1대두포랍정、선봉필등15충항생소민감，대분서청매소화청매소G내약。
A bacteria strain GX-1 was isolated from the liver tissue of the dying Ictalurus punctatus with typical symptoms of intussusceptions.The strain was identified as Aeromonas sobria by an API 20NE test.Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain shared the highest similarity (99.8%) with A.sobria NCIMB 12065.The strain was highly pathogenic in both young and adult healthy I.punctatus, and the symptoms of the artificially infected fish were consistent with the naturally infected fish .Drug sensitivity test results showed that the strain GX-1 was sensitive to 15 types of antibiotics including ofloxacin and sulfamethoxazole , but resistant to prostaphlin and penicillin G .It is the first time in China that A.sobria has been isolated from I.punctatus with intussusceptions in this study .