CAJ | 학술논문

采用线粒体COI和Cyt b基因序列分析相结合的方法，对伊犁河和塔里木河水系6个斑重唇鱼（ Diptychus maculates）群体遗传差异和分化水平进行了比较研究。 AMOVA分析结果显示，斑重唇鱼群体间遗传差异显著大于群体内，群体平均遗传分化指数ΦST分别为0.9802（ COI）和0.8863（ Cyt b），除伊犁河水系两群体差异不显著外（ P＞0.05），其他两两群体间遗传差异均显著。基于Kimura双参数法计算群体间平均遗传距离发现，不仅两水系间群体存在差异，塔里木河水系内部群体也产生了较大的遗传分化。单倍型网络图和系统关系树显示库玛拉克河群体存在两组不同单倍型类群，分别与伊犁河和塔里木河水系单倍型聚类。根据分子钟假说，基于Cyt b基因序列推算斑重唇鱼两支系发生分歧的时间大约在2.41 Ma年的上新世末到更新世初。天山第四纪构造运动第一幕可能是两水系斑重唇鱼发生遗传分化的重要原因，而塔里木河水系群体发生遗传分化可能与塔克拉玛干沙漠的阻隔有关。
채용선립체COI화Cyt b기인서렬분석상결합적방법，대이리하화탑리목하수계6개반중진어（ Diptychus maculates）군체유전차이화분화수평진행료비교연구。 AMOVA분석결과현시，반중진어군체간유전차이현저대우군체내，군체평균유전분화지수ΦST분별위0.9802（ COI）화0.8863（ Cyt b），제이리하수계량군체차이불현저외（ P＞0.05），기타량량군체간유전차이균현저。기우Kimura쌍삼수법계산군체간평균유전거리발현，불부량수계간군체존재차이，탑리목하수계내부군체야산생료교대적유전분화。단배형망락도화계통관계수현시고마랍극하군체존재량조불동단배형류군，분별여이리하화탑리목하수계단배형취류。근거분자종가설，기우Cyt b기인서렬추산반중진어량지계발생분기적시간대약재2.41 Ma년적상신세말도경신세초。천산제사기구조운동제일막가능시량수계반중진어발생유전분화적중요원인，이탑리목하수계군체발생유전분화가능여탑극랍마간사막적조격유관。
In this study, the mitochondrial COI and Cyt b gene sequences were analyzed to reveal the genetic diversity and divergence level in six Diptychus maculatus populations .AMOVA analysis showed that genetic variation among populations was significantly greater than that within populations , and the average genetic differentiation index ΦST were 0.980 2 ( COI) and 0.886 3 ( Cyt b) respectively.Genetic differences between two populations were significant except two popula-tions from Ili River ( P>0.05 ).Average genetic distance based on Kimura two-parameter suggested that not only popula-tions between two river systems showed significant genetic differentiations but certain populations among Tarim River also showed differentiations.Haplotype network charts and phylogenetic trees both revealed Kumalake River population existed two different haplogroups.According to the molecular clock hypothesis , phylogenetic divergence time was about 2.41 Ma when it was late Pliocene to early Pleistocene.The Quaternary tectonic movement in the first act of Tianshan Mountain might be the most important reason for genetic divergence in D.maculates populations from two water systems.However , the ge-netic divergence in D.maculates populations from Tarim River might be caused by the separation of Taklimakan Desert.