淡水渔业
淡水漁業
담수어업
FRESHWATER FISHERIES
2014年
4期
7-12
,共6页
杨建%徐伟%耿龙武%关海红%党云飞%姜海峰
楊建%徐偉%耿龍武%關海紅%黨雲飛%薑海峰
양건%서위%경룡무%관해홍%당운비%강해봉
幼鱼%盐度%半致死浓度%鳃%肾
幼魚%鹽度%半緻死濃度%鰓%腎
유어%염도%반치사농도%새%신
juveniles salinity%LC50%gill%kidney
对大鳞鲃( Barbus capito)、草鱼( Ctenopharyngodon idellus)、松浦镜鲤( Cyprinus carpio Songpu)、黑龙江野鲫( Carassius auratus)和鲢( Hypophthalmichthys molitrix)5种幼鱼进行96 h急性盐度毒性试验,研究了盐度对5种幼鱼的半致死浓度,及其对鳃和肾脏的组织结构影响。结果显示,5种幼鱼盐度96 h半致死浓度( LC50)分别为12.063、10.322、11.780、12.752和9.654 g/L。5种幼鱼的盐度耐受能力顺序为:鲫>大鳞鲃>松浦镜鲤>草鱼>鲢。观察了盐度对受试鱼类鳃和肾脏的组织切片,结果显示:在盐度胁迫下,受试鱼类鳃小片缩短,细胞间隙增大,鳃小片上皮细胞出现不同程度的水肿,甚至脱落;而肾组织中肾小管出现不同程度的萎缩,肾小管管腔变大。
對大鱗鲃( Barbus capito)、草魚( Ctenopharyngodon idellus)、鬆浦鏡鯉( Cyprinus carpio Songpu)、黑龍江野鯽( Carassius auratus)和鰱( Hypophthalmichthys molitrix)5種幼魚進行96 h急性鹽度毒性試驗,研究瞭鹽度對5種幼魚的半緻死濃度,及其對鰓和腎髒的組織結構影響。結果顯示,5種幼魚鹽度96 h半緻死濃度( LC50)分彆為12.063、10.322、11.780、12.752和9.654 g/L。5種幼魚的鹽度耐受能力順序為:鯽>大鱗鲃>鬆浦鏡鯉>草魚>鰱。觀察瞭鹽度對受試魚類鰓和腎髒的組織切片,結果顯示:在鹽度脅迫下,受試魚類鰓小片縮短,細胞間隙增大,鰓小片上皮細胞齣現不同程度的水腫,甚至脫落;而腎組織中腎小管齣現不同程度的萎縮,腎小管管腔變大。
대대린파( Barbus capito)、초어( Ctenopharyngodon idellus)、송포경리( Cyprinus carpio Songpu)、흑룡강야즉( Carassius auratus)화련( Hypophthalmichthys molitrix)5충유어진행96 h급성염도독성시험,연구료염도대5충유어적반치사농도,급기대새화신장적조직결구영향。결과현시,5충유어염도96 h반치사농도( LC50)분별위12.063、10.322、11.780、12.752화9.654 g/L。5충유어적염도내수능력순서위:즉>대린파>송포경리>초어>련。관찰료염도대수시어류새화신장적조직절편,결과현시:재염도협박하,수시어류새소편축단,세포간극증대,새소편상피세포출현불동정도적수종,심지탈락;이신조직중신소관출현불동정도적위축,신소관관강변대。
Experiments were conducted to explore the half lethal concentration ( LC50 ) of salinity and the impact on gill and kidney tissue in 5 juveniles through 96 h acute salinity toxicity test with fresh water test for comparison .According to the results of the acute salinity experiment , the LC50 of salinity in Barbus capito, Ctenopharyngodon idllus, Cyprinus carpio Songpu, Carassius auratus and Hypophthalmichthys molitrix were 12.063 g/L, 10.322 g/L, 11.780 g/L, 12.752 g/L, 9.654 g/L, 9.672 g/L, respectively.Therefore, the sequence of salt-tolerance were: Crucian carp>Barbus capi-to>Songpu mirror carp >grass carp>silver carp.Furthermore, the gill and kidney structure of 5 species juveniles were observed under different salinity treatment by using the technology of histological photomicrography .The results showed that, compared to control group, gill lamellae narrowed, intercellular space increased and the epithelial cells appeared e-dema to different degrees , and even fell off; renal tubules atrophied to different degree and the tubular lumen expanded in the treatment under salinity challenge .