CAJ | 학술논문

目的：探讨半翼基因（ wings apart-like，Wapl ）蛋白在实验鼠粗线期的卵细胞中联会复合体（synaptonemal complex，SC）的表达位置。方法从受孕18.5d胎鼠的卵巢中分离出处于粗线期的卵细胞，然后用抗-联合复合体蛋白2（anti-synaptonemal complex protein 2，SYCP2）和抗-Wapl蛋白进行双免疫染色。结果 SYCP2和Wapl在所检查粗线期的卵细胞位置重叠。结论 Wapl可能是粗线期卵细胞SC的一部分。
목적：탐토반익기인（ wings apart-like，Wapl ）단백재실험서조선기적란세포중련회복합체（synaptonemal complex，SC）적표체위치。방법종수잉18.5d태서적란소중분리출처우조선기적란세포，연후용항-연합복합체단백2（anti-synaptonemal complex protein 2，SYCP2）화항-Wapl단백진행쌍면역염색。결과 SYCP2화Wapl재소검사조선기적란세포위치중첩。결론 Wapl가능시조선기란세포SC적일부분。
Objective In order to analyze the correlation of the wings apart-like( Wapl ) protein and the synaptonemal complex( SC)of pachytene oocytes. Methods The pachytene oocytes were isolated from foetal mice ovaries at 18 . 5 d and double immunostained with anti-synaptonemal complex protein2( SYCP2 )and anti-Wapl. Results In the pachytene oocytes examined,mouse Wapl was colocalized with SYCP2 on the SC. Conclusion Wapl may be a part of pachytene oocyte SC.