CAJ | 학술논문

目的：研究依达拉奉对实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis ， EAE）小鼠的抑制作用。方法将60只雌性C57BL/6小鼠随机分为3组：正常对照组、EAE组、EAE＋依达拉奉组，每组20只。正常对照组用PBS作为对照。后两组应用髓鞘少突胶质细胞糖蛋白35-55、完全福氏佐剂（ CFA）、结核分枝杆菌，制成的抗原乳剂进行造模。免疫2 d后，EAE＋依达拉奉组腹腔注射依达拉奉5 mg/（ kg· d）。3组均监测20 d，每日称重，观察小鼠的摄食和发病情况，并进行神经功能评分。20 d后取小鼠脊髓组织，进行病理学观察。结果 EAE组小鼠的发病率（80％）高于EAE＋依达拉奉组（45％），差异有统计学意义（P＜0．05）。 EAE＋依达拉奉组神经功能评分各个时间点都低于EAE组，差异有统计学意义（P＜0．05），其中，监测10 d后，EAE组神经功能评分（3．6±0．4）级；而EAE＋依达拉奉组评分为（1．3±0．5）级。 HE染色后发现，EAE组小鼠的脊髓内有大量的炎性细胞浸润，白质脱髓鞘改变明显。而EAE＋依达拉奉组小鼠有较少的炎性细胞浸润，且没有白质脱髓鞘改变。结论依达拉奉对EAE的保护作用可能与其清除自由基、减轻炎性反应有关。
목적：연구의체랍봉대실험성자신면역성뇌척수염（experimental autoimmune encephalomyelitis ， EAE）소서적억제작용。방법장60지자성C57BL/6소서수궤분위3조：정상대조조、EAE조、EAE＋의체랍봉조，매조20지。정상대조조용PBS작위대조。후량조응용수초소돌효질세포당단백35-55、완전복씨좌제（ CFA）、결핵분지간균，제성적항원유제진행조모。면역2 d후，EAE＋의체랍봉조복강주사의체랍봉5 mg/（ kg· d）。3조균감측20 d，매일칭중，관찰소서적섭식화발병정황，병진행신경공능평분。20 d후취소서척수조직，진행병이학관찰。결과 EAE조소서적발병솔（80％）고우EAE＋의체랍봉조（45％），차이유통계학의의（P＜0．05）。 EAE＋의체랍봉조신경공능평분각개시간점도저우EAE조，차이유통계학의의（P＜0．05），기중，감측10 d후，EAE조신경공능평분（3．6±0．4）급；이EAE＋의체랍봉조평분위（1．3±0．5）급。 HE염색후발현，EAE조소서적척수내유대량적염성세포침윤，백질탈수초개변명현。이EAE＋의체랍봉조소서유교소적염성세포침윤，차몰유백질탈수초개변。결론의체랍봉대EAE적보호작용가능여기청제자유기、감경염성반응유관。
Objective To study the inhibiting effect of edaravone on experimental autoimmune encephalomyelitis ( EAE ) mouse model.Methods Sixty female C57BL /6 mice were randomly divided into three groups: normal control group, EAE group, EAE +edaravone group (n=20), normal control group with PBS as a control .The application of myelin oligodendrocytes glial cells glycoprotein 35-55 and complete Freund ’ s adjuvant ( CFA) and Mycobacterium tuberculosis antigens emulsions were made modeling in the later two groups .After 2 days immunization , EAE+edaravone group were injected at a dose of 5 mg/( kg· d) of edaravone .Three groups were monitored 20 days, daily weighing, feeding and incidence were observed , and neurological function was scored .Mouse spinal cord tissue was obtained for pathological examination after 20 days.Results In EAE+edaravone treated mice , compared with EAE mice, the incidence and neurological function scores were significantly low (P<0.05).The incidence in EAE group was 80%, while the incidence in EAE +edaravone group mice was 45%.Monitored for 10 days, in EAE group mice, neural function score was 3.6 ±0.4, while in EAE+edaravone group mice neural function score was 1.3 ±0.5.HE staining found a large number of inflamma-tory cell infiltration in EAE mice spinal cord , and white matter demyelination was obvious .Nevertheless edaravone group mice had fe-wer inflammatory cell infiltration , and no white matter demyelination .Conclusions Protective effect of edaravone on EAE is related to scavenging free radicals and reducing inflammation .