医学美学美容（中旬刊）
醫學美學美容（中旬刊）
의학미학미용（중순간）
MEDICAL AESTHETICS AND COSMETOLOGY
2014年
7期
54-55
,共2页
叶俊华%解士海%杨观招
葉俊華%解士海%楊觀招
협준화%해사해%양관초
氢化可的松%黑素细胞%角质形成细胞%共培养
氫化可的鬆%黑素細胞%角質形成細胞%共培養
경화가적송%흑소세포%각질형성세포%공배양
Hydrocrtison%Melanocte%Keratinocyte%Co-culture
目的：研究氢化可的松对人表皮共培养体系（黑素细胞与角质形成细胞）酪氨酸酶活性及黑素生成的影响。方法培养人表皮黑素细胞和人表皮黑素细胞与角质形成细胞共培养体系，利用四甲基偶氮唑蓝（MTT）还原法测定细胞活力，测定酪氨酸酶活性，475nm 比色法测定黑素含量。结果10-7mol/L、10-6μmol/L和10-5mol/L氢化可的松对于黑素细胞及共培养细胞酪氨酸酶活性及黑素生成均有较强的剂量相关性增强作用，10-6μmol/L和10-5mol/L氢化可的松与对照组相比有显著差异（p＜0．01）。结论氢化可的松对于共培养体系酪氨酸酶活性及黑素生成有较强的剂量相关性增强作用。
目的：研究氫化可的鬆對人錶皮共培養體繫（黑素細胞與角質形成細胞）酪氨痠酶活性及黑素生成的影響。方法培養人錶皮黑素細胞和人錶皮黑素細胞與角質形成細胞共培養體繫，利用四甲基偶氮唑藍（MTT）還原法測定細胞活力，測定酪氨痠酶活性，475nm 比色法測定黑素含量。結果10-7mol/L、10-6μmol/L和10-5mol/L氫化可的鬆對于黑素細胞及共培養細胞酪氨痠酶活性及黑素生成均有較彊的劑量相關性增彊作用，10-6μmol/L和10-5mol/L氫化可的鬆與對照組相比有顯著差異（p＜0．01）。結論氫化可的鬆對于共培養體繫酪氨痠酶活性及黑素生成有較彊的劑量相關性增彊作用。
목적：연구경화가적송대인표피공배양체계（흑소세포여각질형성세포）락안산매활성급흑소생성적영향。방법배양인표피흑소세포화인표피흑소세포여각질형성세포공배양체계，이용사갑기우담서람（MTT）환원법측정세포활력，측정락안산매활성，475nm 비색법측정흑소함량。결과10-7mol/L、10-6μmol/L화10-5mol/L경화가적송대우흑소세포급공배양세포락안산매활성급흑소생성균유교강적제량상관성증강작용，10-6μmol/L화10-5mol/L경화가적송여대조조상비유현저차이（p＜0．01）。결론경화가적송대우공배양체계락안산매활성급흑소생성유교강적제량상관성증강작용。
Objective To study the effects of hydrocrtison on the tyrosinase activity and melanogenesis in co-culture model of human melanocytes and keratinocytes.Methods The model of co-culture of human melanocytes and keratinocytes was cultured and MTT was used to measure the proliferation of melanocytes and the co-cultures.The tyrosinase activity and melanin con-tent were measured.Results The melanin synthesis and tyrosinase activity were clearly enhanced by hydrocrtison in a dose-dependent manner at concentrations of 10 -7mol/L、10 -6μmol/Land 10-5mol/L in both melanocytes and co-cultures.10 -6μmol/Land 10 -5mol/L of hydrocrtison showed statistical y significant suppression (p <0.01)than the control.Conclusion Hydrocrtison exhibits a dose-dependent enhancing ef ects on melanin synthesis and tyrosinase activity in co-cultures of human melanocytes and keratinocytes.
