CAJ | 학술논문

为建立测定氟苯尼考制剂中非法添加的磺胺二甲嘧啶（ SM2）和磺胺间甲氧嘧啶（ SMM）高效液相色谱分析方法，本研究使用C18色谱柱，以乙腈-0．2％冰醋酸溶液（27∶73）作为流动相进行等度洗脱（流速1．0 mL/min）；采用二极管阵列检测器，检测波长270 nm；进样量20μL。结果表明，磺胺二甲嘧啶（SM2）和磺胺间甲氧嘧啶（SMM）分别在进样浓度为31．25～500．00μg/mL和12．50～200．00μg/mL范围内呈良好线性关系（r2分别为0．9996和1．0000）；平均回收率分别为98．6％（SM2）和99．8％（SMM）；检出限分别为0．125μg/mL（ SM2）和0．225μg/mL（ SMM）。建立的方法简便、准确、可行，适用于氟苯尼考制剂中非法添加的磺胺二甲嘧啶和磺胺间甲氧嘧啶的检测。
위건립측정불분니고제제중비법첨가적광알이갑밀정（ SM2）화광알간갑양밀정（ SMM）고효액상색보분석방법，본연구사용C18색보주，이을정-0．2％빙작산용액（27∶73）작위류동상진행등도세탈（류속1．0 mL/min）；채용이겁관진렬검측기，검측파장270 nm；진양량20μL。결과표명，광알이갑밀정（SM2）화광알간갑양밀정（SMM）분별재진양농도위31．25～500．00μg/mL화12．50～200．00μg/mL범위내정량호선성관계（r2분별위0．9996화1．0000）；평균회수솔분별위98．6％（SM2）화99．8％（SMM）；검출한분별위0．125μg/mL（ SM2）화0．225μg/mL（ SMM）。건립적방법간편、준학、가행，괄용우불분니고제제중비법첨가적광알이갑밀정화광알간갑양밀정적검측。
A detection method of SM2 and SMM illegally adulterated in florfenicol preparations were es-tablished by high performance liquid chromatography ( HPLC) .The chromatography was performed with C 18 column at 270 nm using PDAD.The mobile phase consisted of a mixture of acetonitrile -0.2%acetic acid (27∶73) at a constant flow rate of 1.0 mL/min.The injection volume was 20 μL.The results showed that there were good linear relations (with r2 as 0.9996 and 1.0000 respectively) when the injection concentration was 31.25~500.00 μg/mL (SM2) and 12.50~200.00 μg/mL (SMM) repectively.The average recovery rate was 98.6% (SM2) and 99.8% (SMM).The detection limit was 0.125 μg/mL(SM2) and 0.225μg/mL( SMM) respectively .This method was simple , accurate and feasible and was suitable for detection of SM2 and SMM illegally adulterated in florfenicol preparations .