CAJ | 학술논문

目的：探索应用玻璃化冻融技术冻融卵裂期胚胎及囊胚的临床效果。方法来源于江西省妇幼保健院生殖中心2012年9月至2014年1月1658例玻璃化冻融移植周期（其中卵裂期胚胎1426周期共2907枚胚胎，囊胚232周期共341枚胚胎），用自制麦管作为胚胎载体及相同的玻璃化方法进行冷冻、复苏，及胚胎移植，观察其复苏存活率、全胚融解率、临床妊娠率、植入率和流产率等。结果卵裂期胚胎复苏率为98.5%，全胚融解率是0.35%。囊胚复苏率为99.7%，全胚融解率是0.43%。卵裂期胚胎的胚胎植入率显著低于囊胚（35.4%vs 49.3%）（P<0.05），平均每周期移植胚胎数显著高于囊胚（2.0±0.3 vs 1.5±0.5）（P<0.05），临床妊娠率低于囊胚（50.7%vs 55.4%），差异无显著性意义，P>0.05，双胎率卵裂期胚胎显著高于囊胚（38.3%vs 28.9%）（P<0.05），早期流产率无显著性差异（6.8%vs 7.8%，P>0.05）。结论使用相同的玻璃化冷冻方法对人类卵裂期胚胎和囊胚可获得理想的冷冻效果。
목적：탐색응용파리화동융기술동융란렬기배태급낭배적림상효과。방법래원우강서성부유보건원생식중심2012년9월지2014년1월1658례파리화동융이식주기（기중란렬기배태1426주기공2907매배태，낭배232주기공341매배태），용자제맥관작위배태재체급상동적파리화방법진행냉동、복소，급배태이식，관찰기복소존활솔、전배융해솔、림상임신솔、식입솔화유산솔등。결과란렬기배태복소솔위98.5%，전배융해솔시0.35%。낭배복소솔위99.7%，전배융해솔시0.43%。란렬기배태적배태식입솔현저저우낭배（35.4%vs 49.3%）（P<0.05），평균매주기이식배태수현저고우낭배（2.0±0.3 vs 1.5±0.5）（P<0.05），림상임신솔저우낭배（50.7%vs 55.4%），차이무현저성의의，P>0.05，쌍태솔란렬기배태현저고우낭배（38.3%vs 28.9%）（P<0.05），조기유산솔무현저성차이（6.8%vs 7.8%，P>0.05）。결론사용상동적파리화냉동방법대인류란렬기배태화낭배가획득이상적냉동효과。
Objective To evaluate the efficacy of vitrified cryopreservation for human embryos in cleavage and blastocyst stages using cryoloop. Methods 1658 vitrified cryopreservation and thawing cycles (1426 cycles including 290,7 embryos in cleavage and 232 cycles including 341 embryos in blastocyst stage respectively),from the hospital IVF center,were divided into two groups,as their stages,I. e. cleavage or blastocyst stage,for the later artifical shrinked must be done prior to be freezed,and then rates for thawing survival,implantation,clinic pregnancy and miscarrage were observed. Results The rates of the thawing survival of the cleavage and blastocyst stage embryos were (98.5%,99.7%) respectively. The rates for implantation and the mean transfer embryo number of the two groups were 35.4%,49.3%,and 2.0±0.3,1.5±0.5 (P<0.05). while their clinical pregnancy rates and miscarryge rates were not significantly different (50.7%vs 55.4%,6.8%vs 7.8%). Conclusion This protocol for vitrified cry-opreservation,can work well and suitable for cleavage stage embryos and blastocyst stage embryos as well.