海洋学报(中文版)
海洋學報(中文版)
해양학보(중문판)
ACTA OCEANOLOGICA SINICA
2014年
8期
49-64
,共16页
陈姣%徐继林%李艳%周成旭%严小军
陳姣%徐繼林%李豔%週成旭%嚴小軍
진교%서계림%리염%주성욱%엄소군
海洋硅藻%挥发性成分%培养阶段%固相微萃取-气相色谱-质谱联用
海洋硅藻%揮髮性成分%培養階段%固相微萃取-氣相色譜-質譜聯用
해양규조%휘발성성분%배양계단%고상미췌취-기상색보-질보련용
marine diatom%volatile components%growth phase%head space solid-phase micro-extraction and gas chromatography-mass spectrometry
本文应用顶空固相微萃取气相色谱-质谱联用技术,对威氏海链藻 Thalassiosira weissflogii 、小新月菱形藻 Nitzschia closterium 、角毛藻Chaetoceros calcitrons 3种硅藻的挥发性成分进行分析。结果表明,在3种硅藻的培养过程中,其挥发性成分除了壬醛及醇类呈现先增后降的趋势外,大多数不饱和醛类的含量在不断增加,烷烯烃类物质在不断减少,而乙酸乙酯、2-甲基-2-丙酸甲酯这类酯类化合物在角毛藻衰败期会显著富集。通过对代表性饵料硅藻的挥发性成分进行研究,可为海水养殖过程中微藻投喂种类、投喂时机的选择提供理论依据,并为海洋硅藻的进一步开发奠定基础。
本文應用頂空固相微萃取氣相色譜-質譜聯用技術,對威氏海鏈藻 Thalassiosira weissflogii 、小新月蔆形藻 Nitzschia closterium 、角毛藻Chaetoceros calcitrons 3種硅藻的揮髮性成分進行分析。結果錶明,在3種硅藻的培養過程中,其揮髮性成分除瞭壬醛及醇類呈現先增後降的趨勢外,大多數不飽和醛類的含量在不斷增加,烷烯烴類物質在不斷減少,而乙痠乙酯、2-甲基-2-丙痠甲酯這類酯類化閤物在角毛藻衰敗期會顯著富集。通過對代錶性餌料硅藻的揮髮性成分進行研究,可為海水養殖過程中微藻投餵種類、投餵時機的選擇提供理論依據,併為海洋硅藻的進一步開髮奠定基礎。
본문응용정공고상미췌취기상색보-질보련용기술,대위씨해련조 Thalassiosira weissflogii 、소신월릉형조 Nitzschia closterium 、각모조Chaetoceros calcitrons 3충규조적휘발성성분진행분석。결과표명,재3충규조적배양과정중,기휘발성성분제료임철급순류정현선증후강적추세외,대다수불포화철류적함량재불단증가,완희경류물질재불단감소,이을산을지、2-갑기-2-병산갑지저류지류화합물재각모조쇠패기회현저부집。통과대대표성이료규조적휘발성성분진행연구,가위해수양식과정중미조투위충류、투위시궤적선택제공이론의거,병위해양규조적진일보개발전정기출。
The volatile components in marine diatoms Thalassiosira weissflogii ,Nitzschia closterium and Chaetoc-eros calcitrons were analyzed by head space solid-phase micro-extraction and gas chromatography-mass spectrome-try.The differences of volatile components among three diatoms at different growth stages were studied by SIM-CA-P software.The results showed that the content of most unsaturated aldehydes increased through the entire culture stages of three diatoms except for a saturated aldehyde,Nonanal,whose content first increased and then de-creased.The content of Alkanes continued to decline.Alcohols also increased first and then decreased.Esters in-cluding acetic acid ethyl ester and 2-methyl-2-Propenoic acid methyl ester were significantly enriched in the decline phase of C.calcitrons.According to the present study on volatile components of these diatoms,we can provide im-portant reference not only for choosing microalgae species and feeding period in marine aquaculture,but also for studying the reason of odor formation and further development and utilization of marine diatom.