CAJ | 학술논문

目的：探讨中药合剂对实验性大鼠结肠炎的微生态失调、免疫功能的影响，寻找治疗溃疡性结肠炎的中药合剂。方法用乙酸诱发大鼠实验性结肠炎模型，筛选具有免疫增强作用的补益类中药制成的常态中药，分别于第7天，第10天，第14天分批剖腹取肠道内容物及结肠病变肠段，培养后观察肠道双歧杆菌、乳酸杆菌、肠杆菌、肠球菌4种正常菌群变化，肉眼及苏木素-伊红（H E ）染色后观察肠黏膜损伤及治疗组的修复情况，同时用免疫组织化学法测定各组T细胞亚群的数量。结果中药0708能扶植肠道正常菌群生长，促进肠黏膜损伤修复，控制肠道菌易位，各项指标与自然恢复组比较差异有统计学意义（ P ＜0．05）。结论此组常态中药可以扶植肠道正常菌群生长，调整肠道微生态失调、修复肠道黏膜损伤、增强免疫功能。
목적：탐토중약합제대실험성대서결장염적미생태실조、면역공능적영향，심조치료궤양성결장염적중약합제。방법용을산유발대서실험성결장염모형，사선구유면역증강작용적보익류중약제성적상태중약，분별우제7천，제10천，제14천분비부복취장도내용물급결장병변장단，배양후관찰장도쌍기간균、유산간균、장간균、장구균4충정상균군변화，육안급소목소-이홍（H E ）염색후관찰장점막손상급치료조적수복정황，동시용면역조직화학법측정각조T세포아군적수량。결과중약0708능부식장도정상균군생장，촉진장점막손상수복，공제장도균역위，각항지표여자연회복조비교차이유통계학의의（ P ＜0．05）。결론차조상태중약가이부식장도정상균군생장，조정장도미생태실조、수복장도점막손상、증강면역공능。
Objective To investigate the influence of Chinese medicine mixture on microecological imbalance and immune function in experimental rat colitis .To explore Chinese medicine agent for the treatment of ulcerative colitis .Methods Acetic acid-induced colitis model in rats were respectively given beneficial traditional Chinese medicines ,bifidobiogen ,or the two-drug combination therapy .Then we harvested intestinal contents and colon with lesion in batches .After culture ,changes in intestinal flora were observed .Meanwhile ,we observed the in-testinal mucosal injury ,recovery of the intestinal mucosa after treatment ,and determined the number of T lym-phocyte subsets in each group.Results Traditional Chinese medicine (TCM)0708 can foster the intestinal normal flora growth ,improve the intestinal mucosa damage and control the intestinal bacterial translocation .Compared with natural recovery group ,the indicators were of significant difference ( P <0 .05) .Conclusion The group of normal Chinese traditional medicine can foster the intestinal normal flora growth ,adjust intestinal microecological imbalance ,repair of the intestinal mucosa damage and enhance the immune function .