中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
28期
4517-4523
,共7页
文军%吴补领%段建民%李洪涛%李斯翰%李鑫
文軍%吳補領%段建民%李洪濤%李斯翰%李鑫
문군%오보령%단건민%리홍도%리사한%리흠
干细胞%培养%富血小板血浆%乳牙牙髓干细胞%间质干细胞%骨髓间充质干细胞%胎牛血清%血小板源性生长因子%细胞增殖%转化生长因子β
榦細胞%培養%富血小闆血漿%乳牙牙髓榦細胞%間質榦細胞%骨髓間充質榦細胞%胎牛血清%血小闆源性生長因子%細胞增殖%轉化生長因子β
간세포%배양%부혈소판혈장%유아아수간세포%간질간세포%골수간충질간세포%태우혈청%혈소판원성생장인자%세포증식%전화생장인자β
platelet-rich plasma%tooth%deciduous%dental pulp%serum albumin%bovine
背景：课题组前期实验发现用液氮冻融激活的改良富血小板血浆可以促进骨髓间充质干细胞和牙髓干细胞的增殖，且这种促增殖作用具有浓度特异性。目的：体外研究不同浓度的改良富血小板血浆对人乳牙牙髓干细胞增殖的影响。方法：利用全自动血细胞分离机制备血小板浓缩物，反复冻融法激活，获得改良富血小板血浆；以α-MEM作为基础培养基，分别加入体积分数为2%，5%，10%，20%4种不同浓度改良富血小板血浆或者体积分数10%胎牛血清进行培养，观察细胞增殖差异。结果与结论：不同浓度的改良富血小板血浆均能够促进乳牙牙髓干细胞增殖，体积分数2%改良富血小板血浆与体积分数10%胎牛血清促进乳牙牙髓干细胞增殖作用接近。提示体积分数2%改良富血小板血浆可以促进乳牙牙髓干细胞的增殖，有可能代替胎牛血清用于乳牙牙髓干细胞的体外扩增。
揹景：課題組前期實驗髮現用液氮凍融激活的改良富血小闆血漿可以促進骨髓間充質榦細胞和牙髓榦細胞的增殖，且這種促增殖作用具有濃度特異性。目的：體外研究不同濃度的改良富血小闆血漿對人乳牙牙髓榦細胞增殖的影響。方法：利用全自動血細胞分離機製備血小闆濃縮物，反複凍融法激活，穫得改良富血小闆血漿；以α-MEM作為基礎培養基，分彆加入體積分數為2%，5%，10%，20%4種不同濃度改良富血小闆血漿或者體積分數10%胎牛血清進行培養，觀察細胞增殖差異。結果與結論：不同濃度的改良富血小闆血漿均能夠促進乳牙牙髓榦細胞增殖，體積分數2%改良富血小闆血漿與體積分數10%胎牛血清促進乳牙牙髓榦細胞增殖作用接近。提示體積分數2%改良富血小闆血漿可以促進乳牙牙髓榦細胞的增殖，有可能代替胎牛血清用于乳牙牙髓榦細胞的體外擴增。
배경：과제조전기실험발현용액담동융격활적개량부혈소판혈장가이촉진골수간충질간세포화아수간세포적증식，차저충촉증식작용구유농도특이성。목적：체외연구불동농도적개량부혈소판혈장대인유아아수간세포증식적영향。방법：이용전자동혈세포분리궤제비혈소판농축물，반복동융법격활，획득개량부혈소판혈장；이α-MEM작위기출배양기，분별가입체적분수위2%，5%，10%，20%4충불동농도개량부혈소판혈장혹자체적분수10%태우혈청진행배양，관찰세포증식차이。결과여결론：불동농도적개량부혈소판혈장균능구촉진유아아수간세포증식，체적분수2%개량부혈소판혈장여체적분수10%태우혈청촉진유아아수간세포증식작용접근。제시체적분수2%개량부혈소판혈장가이촉진유아아수간세포적증식，유가능대체태우혈청용우유아아수간세포적체외확증。
BACKGROUND:Previous experiments have shown that modified platelet-rich plasma activated by liquid nitrogen freezing and thawing can promote the proliferation of human bone marrow mesenchymal stem cells and dental pulp stem cells in a concentration-dependent manner. OBJECTIVE:To investigate the effect of modified platelet-rich plasma at different concentrations on the proliferation of dental pulp stem cells from human exfoliated deciduous teeth. METHODS:Platelets were selected and harvested by automatic blood cellanalyzer, and then activated by liquid nitrogen freezing and thawing.α-MEM served as basal medium. Different concentrations of modified platelet-rich plasma (2%, 5%, 10%, 20%) or 10%fetal bovine serum were added, respectively. The difference in cellproliferation was observed. RESULTS AND CONCLUSION:Modified platelet-rich plasma at different concentrations could promote the proliferation of dental pulp stem cells from deciduous teeth. The effects of 2%modified platelet-rich plasma and 10%fetal bovine serum on promoting the proliferation of dental pulp stem cells from deciduous teeth were similar. These results indicated that 2%modified platelet-rich plasma could promote the proliferation of dental pulp stem cells from deciduous teeth, and substitute for fetal bovine serum in the amplification of dental pulp stem cells from deciduous teeth in vitro.
