CAJ | 학술논문

背景：牙周膜干细胞生物作用是目前牙周病治疗研究的热点，牙周膜成纤维细胞是其分化的终末功能细胞之一，也是其主要的支持细胞，两者生物学特性的差异研究鲜有报道。目的：比较牙周膜干细胞与牙周膜细胞生物学特性的差异。方法：用组织块法体外对牙周膜细胞以及单细胞克隆分离纯化后的人牙周膜干细胞两种细胞分别进行显微镜下形态观察，CCK8法检测并绘制2种细胞的生长曲线。流式细胞分析比较2种细胞的细胞周期以及细胞表面标记物的表达、实时PCR对2种细胞碱性磷酸酶、增殖细胞核抗原和Scleraxis基因进行检测。结果与结论：牙周膜干细胞与牙周膜成纤维细胞外观差别明显，人牙周膜干细胞的生长曲线培养前5d要低于牙周膜细胞，但在5 d后明显高于牙周膜细胞。人牙周膜干细胞与牙周膜细胞的细胞周期分别为41.1%和23.9%。表面标记物检测结果显示2种细胞虽有相似的表达，但在表达率差异有有显著性意义。实时荧光定量PCR结果显示，人牙周膜干细胞在碱性磷酸酶、增殖细胞核抗原以及Scleraxis基因的表达检测均高于牙周膜细胞。表明牙周膜干细胞在成骨增殖等生物学功能上比牙周膜细胞具有更强的潜能。
배경：아주막간세포생물작용시목전아주병치료연구적열점，아주막성섬유세포시기분화적종말공능세포지일，야시기주요적지지세포，량자생물학특성적차이연구선유보도。목적：비교아주막간세포여아주막세포생물학특성적차이。방법：용조직괴법체외대아주막세포이급단세포극륭분리순화후적인아주막간세포량충세포분별진행현미경하형태관찰，CCK8법검측병회제2충세포적생장곡선。류식세포분석비교2충세포적세포주기이급세포표면표기물적표체、실시PCR대2충세포감성린산매、증식세포핵항원화Scleraxis기인진행검측。결과여결론：아주막간세포여아주막성섬유세포외관차별명현，인아주막간세포적생장곡선배양전5d요저우아주막세포，단재5 d후명현고우아주막세포。인아주막간세포여아주막세포적세포주기분별위41.1%화23.9%。표면표기물검측결과현시2충세포수유상사적표체，단재표체솔차이유유현저성의의。실시형광정량PCR결과현시，인아주막간세포재감성린산매、증식세포핵항원이급Scleraxis기인적표체검측균고우아주막세포。표명아주막간세포재성골증식등생물학공능상비아주막세포구유경강적잠능。
BACKGROUND:The biological function of human periodontal ligament stem cells is a hot area of research in the treatment of periodontal disease. Human periodontal ligament cells are one of the end cells derived from human periodontal ligament stem cells;meanwhile, it can also provide supports to the development of human periodontal ligament stem cells. However, few studies are reported about the difference of biological characteristics between human periodontal ligament stem cells and human periodontal ligament cells. OBJECTIVE:To compare the differences of biological characteristics between human periodontal ligament stem cells and human periodontal ligament cells. METHODS:The human periodontal ligament stem cells and human periodontal ligament cells were isolated and purified using tissue explant method and cellclone method, respectively, and then were observed under light microscope to compare the differences of morphology. cellproliferation curves of human periodontal ligament stem cells and human periodontal ligament cells were drawn respectively with cellcounting kit 8 assay. Flow cytometry analysis was used to detect their cellcircles and their surface markers expressions. The alkaline phosphatase gene, proliferating cellnuclear antigen gene and Scleraxis gene of human periodontal ligament stem cells and human periodontal ligament cells were detected by Real-time PCR assay.RESULTS AND CONCLUSION:The human periodontal ligament stem cells and human periodontal ligament cells showed a notable difference in morphology under the light microscope observation. During the first 5 days, the cellproliferation curve of human periodontal ligament stem cells was lower than that of human periodontal ligament cells, but 5 days later, the curve of human periodontal ligament stem cells was significantly higher than that of human periodontal ligament cells. The cellcircles of human periodontal ligament stem cells and human periodontal ligament cells were 41.1%and 23.9%, respectively. The surface markers of human periodontal ligament stem cells and human periodontal ligament cells were similar, but their expression rates had significant difference. The expressions of alkaline phosphatase gene, proliferating cellnuclear antigen gene and Scleraxis gene of human periodontal ligament stem cells were significantly higher than those of human periodontal ligament cells. The above results suggest that human periodontal ligament stem cells have much stronger potential ability than human periodontal ligament cells in osteogensis and cellproliferation.