CAJ | 학술논문

背景：研究发现外源性骨髓间充质干细胞能够定居于肺脏组织，参与肺组织的再生，但其修复衰老肺损伤方面的报道较少。目的：观察骨髓间充质干细胞减轻D-半乳糖所致肺脏组织损伤的作用。方法：选择SD大鼠30只，随机分为3组，包括对照组、衰老模型组、细胞治疗组，每组10只。衰老模型组和细胞治疗组大鼠每日皮下注射D-半乳糖，连续4个月制备衰老模型。细胞治疗组通过尾静脉输注3×106个骨髓间充质干细胞，每周1次，连续4周；对照组和衰老模型组给予同等剂量的细胞培养液。采用表达绿色荧光蛋白的慢病毒载体标记骨髓间充质干细胞，以确定骨髓间充质干细胞在大鼠肺脏的植入情况。测定3组大鼠肺脏组织中超氧化物歧化酶活性和丙二醛水平；苏木精-伊红染色观察3组大鼠肺脏组织结构的差异。结果与结论：绿色荧光蛋白标记的骨髓间充质干细胞移植给大鼠后，能向肺脏组织迁移并存活。与衰老模型组相比，细胞治疗组肺脏的超氧化物歧化酶活性明显升高，丙二醛水平明显降低。各组大鼠的组织病理切片显示，模型组大鼠的正常肺泡结构破坏，出现气腔扩大、肺气肿改变；而细胞治疗组大鼠的肺脏损伤有明显修复。结果可见骨髓间充质干细胞对衰老大鼠的肺脏损伤有修复作用，从而发挥其抗衰老作用。
배경：연구발현외원성골수간충질간세포능구정거우폐장조직，삼여폐조직적재생，단기수복쇠로폐손상방면적보도교소。목적：관찰골수간충질간세포감경D-반유당소치폐장조직손상적작용。방법：선택SD대서30지，수궤분위3조，포괄대조조、쇠로모형조、세포치료조，매조10지。쇠로모형조화세포치료조대서매일피하주사D-반유당，련속4개월제비쇠로모형。세포치료조통과미정맥수주3×106개골수간충질간세포，매주1차，련속4주；대조조화쇠로모형조급여동등제량적세포배양액。채용표체록색형광단백적만병독재체표기골수간충질간세포，이학정골수간충질간세포재대서폐장적식입정황。측정3조대서폐장조직중초양화물기화매활성화병이철수평；소목정-이홍염색관찰3조대서폐장조직결구적차이。결과여결론：록색형광단백표기적골수간충질간세포이식급대서후，능향폐장조직천이병존활。여쇠로모형조상비，세포치료조폐장적초양화물기화매활성명현승고，병이철수평명현강저。각조대서적조직병리절편현시，모형조대서적정상폐포결구파배，출현기강확대、폐기종개변；이세포치료조대서적폐장손상유명현수복。결과가견골수간충질간세포대쇠로대서적폐장손상유수복작용，종이발휘기항쇠로작용。
BACKGROUND:Studies have shown that exogenous bone marrow mesenchymal stem cells can settle down in lung tissue, participate in long regeneration, but few studies concerned the repair of aging lung injury. OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cells on lung injury induced by D-galactose. METHODS:A total of 30 Sprague-Dawley rats were equal y divided into three groups at random:control group, aging model group and celltreatment group. To establish the aging rats, 10 rats each in the aging model group and celltreatment group were daily subcutaneously injected with D-galactose for 4 months. 3×106 bone marrow mesenchymal stem cells were transplanted via caudal vein in the celltreatment group, once a week, for 4 weeks. cellmedium of equal dose was added in the control and aging model groups. Bone marrow mesenchymal stem cells were transfected by lentiviral vectors expressing green fluorescent protein to determine the implantation of bone marrow mesenchymal stem cells in rat lung. Superoxide dismutase activity and malondialdehyde content in rat lung were measured in each group. The difference in rat lung structure was observed using hematoxylin-eosin staining in each group. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells marked by green fluorescent protein were implanted in rats, migrated towards lung tissue and survived. Compared with aging model group, superoxide dismutase activity was apparently increased, but malondialdehyde content was obviously diminished in the celltreatment group. In each group, histopathological sections revealed that normal pulmonary alveolus was damaged in the aging model group, showing enlarged air cavity and emphysema. Lung injury was evidently repaired inthe celltreatment group. Results suggested that bone marrow mesenchymal stem cells could repair lung injury in aging rats, and exert anti-aging effects.